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홍성유

Hong, Sung You
Synthetic Organic Chemistry Lab.
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Alkylation of nucleobases by 2-chloro-N,N-diethylethanamine hydrochloride (CDEAH) sensitizes PARP1-deficient tumors

Author(s)
Wie, MinwooKhim, Keon WooGroehler Iv, Arnold SHeo, SoominWoo, JunhyeokSon, KookLee, Eun ARa, Jae SunHong, Sung YouScharer, Orlando D.Choi, Jang HyunMyung, Kyungjae
Issued Date
2023-09
DOI
10.1093/narcan/zcad042
URI
https://scholarworks.unist.ac.kr/handle/201301/65706
Citation
NAR Cancer, v.5, no.3, pp.zcad042
Abstract
Targeting BRCA1-and BRCA2-deficient tumors through synthetic lethality using poly(ADP-ribose) polymerase inhibitors (PARPi) has emerged as a successful strategy for cancer therapy. PARPi monotherapy has shown excellent efficacy and safety profiles in clinical practice but is limited by the need for tumor genome mutations in BRCA or other homologous recombination genes as well as the rapid emergence of resistance. In this study, we identified 2-chloro-N,N-diethylethanamine hydrochloride (CDEAH) as a small molecule that selectively kills PARP1-and xeroderma pigmentosum A-deficient cells. CDEAH is a monofunctional alkylating agent that preferentially alkylates guanine nucleobases, forming DNA adducts that can be removed from DNA by either a PARP1-dependent base excision repair or nucleotide excision repair. Treatment of PARP1-deficient cells leads to the formation of strand breaks, an accumulation of cells in S phase and activation of the DNA damage response. Furthermore, CDEAH selectively inhibits PARP1-deficient xenograft tumor growth compared to isogenic PARP1-proficient tumors. Collectively, we report the discovery of an alkylating agent inducing DNA damage that requires PARP1 activity for repair and acts synergistically with PARPi.
Publisher
Oxford University Press
ISSN
2632-8674

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