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Jang, Jaesung
Sensors & Aerosols Lab
Research Interests
  • Bio-MEMS
  • Bioaerosols
  • Sensor
  • Aerosol Cleaning
  • Fluid Mechanics at microscales

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Mechanical desorption of immobilized proteins using carbon dioxide aerosols for reusable biosensors

Cited 2 times inthomson ciCited 2 times inthomson ci
Title
Mechanical desorption of immobilized proteins using carbon dioxide aerosols for reusable biosensors
Author
Singh, RenuHong, SeongkyeolJang, Jaesung
Issue Date
2015-01
Publisher
ELSEVIER SCIENCE BV
Citation
ANALYTICA CHIMICA ACTA, v.853, pp.588 - 595
Abstract
effective desorption of probe molecules. We present a novel mechanical desorption technique to reuse biosensors by using periodic jets of carbon dioxide (CO2) aerosols (a mixture of solid and gaseous CO2), and demonstrate its feasibility by removing physically adsorbed and covalently bonded fluorescent proteins i.e., Escherichia coli fluorescein isothiocyanate antibody and bovine serum albumin (E. coli FITC-Ab and FITC-BSA) from silicon chips. The proteins on the chip surfaces were measured by fluorescent images before and after applying the aerosols. The removal efficiency of the aerosol treatment was measured for various concentrations (1-20 mg mL(-1)) of E. coli FITC-Ab and FITC-BSA with two different removal cycles (5 and 11 cycles; each cycle: 8 s). We observed high removal efficiencies (>93.5% for physically adsorbed Ab and >84.6% for covalently bonded Ab) at 11 cycle aerosol treatment. This CO2 aerosol treatment did not undermine re-functionalization, which was confirmed by the fluorescent images of FITC-Abs for fresh and reused chips. Desorption of the immobilized layers was validated by Fourier transform infrared and X-ray photoelectron spectroscopic analyses. We also conducted an experiment on the regeneration of E. coli sensing chips using this aerosol treatment, and the chips were re-used 5 times successfully. This mechanical desorption technique is a highly effective and novel strategy for reusable biosensors. (C) 2014 Elsevier B.V. All rights reserved.
URI
https://scholarworks.unist.ac.kr/handle/201301/9925
URL
http://www.sciencedirect.com/science/article/pii/S0003267014013191
DOI
10.1016/j.aca.2014.11.006
ISSN
0003-2670
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