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Jang, Jaesung
Sensors & Aerosols Lab
Research Interests
  • Bio-MEMS
  • Bioaerosols
  • Sensor
  • Aerosol Cleaning
  • Fluid Mechanics at microscales


Mechanical desorption of immobilized proteins using carbon dioxide aerosols for reusable biosensors

DC Field Value Language Singh, Renu ko Hong, Seongkyeol ko Jang, Jaesung ko 2015-01-08T00:04:03Z - 2015-01-07 ko 2015-01 ko
dc.identifier.citation ANALYTICA CHIMICA ACTA, v.853, pp.588 - 595 ko
dc.identifier.issn 0003-2670 ko
dc.identifier.uri -
dc.description.abstract effective desorption of probe molecules. We present a novel mechanical desorption technique to reuse biosensors by using periodic jets of carbon dioxide (CO2) aerosols (a mixture of solid and gaseous CO2), and demonstrate its feasibility by removing physically adsorbed and covalently bonded fluorescent proteins i.e., Escherichia coli fluorescein isothiocyanate antibody and bovine serum albumin (E. coli FITC-Ab and FITC-BSA) from silicon chips. The proteins on the chip surfaces were measured by fluorescent images before and after applying the aerosols. The removal efficiency of the aerosol treatment was measured for various concentrations (1-20 mg mL(-1)) of E. coli FITC-Ab and FITC-BSA with two different removal cycles (5 and 11 cycles; each cycle: 8 s). We observed high removal efficiencies (>93.5% for physically adsorbed Ab and >84.6% for covalently bonded Ab) at 11 cycle aerosol treatment. This CO2 aerosol treatment did not undermine re-functionalization, which was confirmed by the fluorescent images of FITC-Abs for fresh and reused chips. Desorption of the immobilized layers was validated by Fourier transform infrared and X-ray photoelectron spectroscopic analyses. We also conducted an experiment on the regeneration of E. coli sensing chips using this aerosol treatment, and the chips were re-used 5 times successfully. This mechanical desorption technique is a highly effective and novel strategy for reusable biosensors. (C) 2014 Elsevier B.V. All rights reserved. ko
dc.description.statementofresponsibility close -
dc.language 영어 ko
dc.publisher ELSEVIER SCIENCE BV ko
dc.title Mechanical desorption of immobilized proteins using carbon dioxide aerosols for reusable biosensors ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84922455229 ko
dc.identifier.wosid 000345652000062 ko
dc.type.rims ART ko
dc.description.wostc 2 *
dc.description.scopustc 2 * 2015-12-28 * 2015-11-04 *
dc.identifier.doi 10.1016/j.aca.2014.11.006 ko
dc.identifier.url ko
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