BROWSE

Related Researcher

Author's Photo

Kim, Yung Sam
Ultrafast 2D IR Spectroscopy Lab
Research Interests
  • 2D IR, chemical exchange of H-bond, fast reaction dynamics, protein dynamics

ITEM VIEW & DOWNLOAD

2D IR provides evidence for mobile water molecules in beta-amyloid fibrils

Cited 73 times inthomson ciCited 32 times inthomson ci
Title
2D IR provides evidence for mobile water molecules in beta-amyloid fibrils
Author
Kim, Yung SamLiu, LiuAxelsen, Paul H.Hochstrasser, Robin M.
Issue Date
2009-10
Publisher
NATL ACAD SCIENCES
Citation
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.106, no.42, pp.17751 - 17756
Abstract
The motion of water molecules close to amide groups causes their vibrational frequencies to vary rapidly in time. These variations are uniquely sensed by 2-dimensional infrared spectroscopy (2D IR). Here, it is proposed from 2-dimensional experiments on fibrils of amyloid β (Aβ)40 that there are water molecules in the fibrils. The spatial locations of the water (D 2O) were inferred from the responses of 18 amide modes of Aβ40 labeled with 13C = 18O. Fast frequency variations were found for residues L17 and V18 and for the apposed residues L34 and V36, suggesting cavities or channels containing mobile water molecules can form between the 2 sheets. Spectroscopic analysis showed that there are 1.2 water molecules per strand in the fibrils. The 13C = 18O substitution of 1 residue per strand creates a linear array of isotopologs along the fibril axis that manifests clearly identifiable vibrational transitions. Here, it is shown from the distributions of amide-I′ vibrational frequencies that the regularity of these chains is strongly residue dependent and in most cases the distorted regions are also those associated with the putative mobile water molecules. It is proposed that Aβ40 fibrils contain structurally significant mobile water molecules within the intersheet region.
URI
https://scholarworks.unist.ac.kr/handle/201301/8835
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=70449584579
DOI
10.1073/pnas.0909888106
ISSN
0027-8424
Appears in Collections:
CHM_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU