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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Serotonin stimulates GnRH secretion through the c-Src-PLC gamma 1 pathway in GT1-7 hypothalamic cells

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Title
Serotonin stimulates GnRH secretion through the c-Src-PLC gamma 1 pathway in GT1-7 hypothalamic cells
Author
Kim, Hyeon SooYumkham, SanatombiChoi, Jang HyunSon, Gi HoonKim, KyungjinRyu, Sung HoSuh, Pann-Ghill
Issue Date
2006-09
Publisher
BIOSCIENTIFICA LTD
Citation
JOURNAL OF ENDOCRINOLOGY, v.190, no.3, pp.581 - 591
Abstract
Serotonin is a neurotransmitter that alters the hypothalamic-pituitary-adrenal axis. To date, however, the molecular mechanisms underlying the role of serotonin in hormone secretion have remained largely unclear. In this study, we report that serotonin activates phospholipase C (PLC) gamma 1 in an Src-dependent manner in hypothalamic GT1-7 cells, and that pretreatment with either 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazole [3, 4-d] pyrimidine, an Src-kinase inhibitor, or U73122, a PLC inhibitor, attenuates the serotonin-induced increase in calcium levels. Also, PLC gamma 1 binds to c-Src through the Src-homology (SH) 223 domain upon serotonin treatment. Moreover, calcium increase is alleviated in the cells transiently expressing SH223 domain-deleted PLC gamma 1 or lipase inactive mutant PLC gamma 1, as compared with cells transfected with wild-type PLC gamma 1. Furthermore, the inhibition of the activities of either PLC or Src results in a significant diminution of the serotonin-induced release of gonadotropin-releasing hormone (GnRH). In addition, the results of our small-interfering RNA experiment confirm that endogenous PLC gamma 1 is a prerequisite for serotonin-mediated signaling pathways. Taken together, our findings demonstrate that serotonin stimulates the release of GnRH through the Src-PLC gamma 1 pathway, via the modulation of intracellular calcium levels.
URI
https://scholarworks.unist.ac.kr/handle/201301/7264
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33749847235
DOI
10.1677/joe.1.06727
ISSN
0022-0795
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