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DC Field | Value | Language |
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dc.citation.endPage | 591 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 581 | - |
dc.citation.title | JOURNAL OF ENDOCRINOLOGY | - |
dc.citation.volume | 190 | - |
dc.contributor.author | Kim, Hyeon Soo | - |
dc.contributor.author | Yumkham, Sanatombi | - |
dc.contributor.author | Choi, Jang Hyun | - |
dc.contributor.author | Son, Gi Hoon | - |
dc.contributor.author | Kim, Kyungjin | - |
dc.contributor.author | Ryu, Sung Ho | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.date.accessioned | 2023-12-22T09:42:54Z | - |
dc.date.available | 2023-12-22T09:42:54Z | - |
dc.date.created | 2014-10-14 | - |
dc.date.issued | 2006-09 | - |
dc.description.abstract | Serotonin is a neurotransmitter that alters the hypothalamic-pituitary-adrenal axis. To date, however, the molecular mechanisms underlying the role of serotonin in hormone secretion have remained largely unclear. In this study, we report that serotonin activates phospholipase C (PLC) gamma 1 in an Src-dependent manner in hypothalamic GT1-7 cells, and that pretreatment with either 4-amino-5-(4-chlorophenyl)-7-(t-butyl) pyrazole [3, 4-d] pyrimidine, an Src-kinase inhibitor, or U73122, a PLC inhibitor, attenuates the serotonin-induced increase in calcium levels. Also, PLC gamma 1 binds to c-Src through the Src-homology (SH) 223 domain upon serotonin treatment. Moreover, calcium increase is alleviated in the cells transiently expressing SH223 domain-deleted PLC gamma 1 or lipase inactive mutant PLC gamma 1, as compared with cells transfected with wild-type PLC gamma 1. Furthermore, the inhibition of the activities of either PLC or Src results in a significant diminution of the serotonin-induced release of gonadotropin-releasing hormone (GnRH). In addition, the results of our small-interfering RNA experiment confirm that endogenous PLC gamma 1 is a prerequisite for serotonin-mediated signaling pathways. Taken together, our findings demonstrate that serotonin stimulates the release of GnRH through the Src-PLC gamma 1 pathway, via the modulation of intracellular calcium levels. | - |
dc.identifier.bibliographicCitation | JOURNAL OF ENDOCRINOLOGY, v.190, no.3, pp.581 - 591 | - |
dc.identifier.doi | 10.1677/joe.1.06727 | - |
dc.identifier.issn | 0022-0795 | - |
dc.identifier.scopusid | 2-s2.0-33749847235 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/7264 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33749847235 | - |
dc.identifier.wosid | 000241324000003 | - |
dc.language | 영어 | - |
dc.publisher | BIOSCIENTIFICA LTD | - |
dc.title | Serotonin stimulates GnRH secretion through the c-Src-PLC gamma 1 pathway in GT1-7 hypothalamic cells | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | EPIDERMAL-GROWTH-FACTOR | - |
dc.subject.keywordPlus | PHOSPHOLIPASE-C-GAMMA | - |
dc.subject.keywordPlus | HORMONE NEURONS | - |
dc.subject.keywordPlus | ANTERIOR HYPOTHALAMUS | - |
dc.subject.keywordPlus | NEUROPEPTIDE-Y | - |
dc.subject.keywordPlus | SRC FAMILY | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | RAT | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
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