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Kang, Byoung Heon
Cancer Biology Lab
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  • Mitochondrial chaperones, cancer biology, cell death, metabolism, cancer stem cells, cancer therapeutics development

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Effects of mutations in the C-terminal region of NIa protease on cis-cleavage between NIa and NIb

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Title
Effects of mutations in the C-terminal region of NIa protease on cis-cleavage between NIa and NIb
Author
Kim, DHHan, JSLew, JSKim, SSKang, Byoung HeonHwang, DCJang, DSKim, WSong, BDChoi, KY
Issue Date
1998-02
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
VIROLOGY, v.241, no.1, pp.94 - 100
Abstract
Mutational analyses were carried out to investigate whether the nuclear inclusion protein a (NIa) C-terminal amino acids of turnip mosaic potyvirus play any roles in the cis-cleavage between NIa and NIb. The processing rate of the NIa-NIb junction sequence was decreased significantly by either V240D or Q243A mutation while little affected by F226D, V228E, K230E, 1232D, or L235D mutation. The mutation of W212S, G213S, or 1217D abolishing the cleavage at the NIb-CP or 6K1-cylindrical inclusion protein junction sequence decreased the processing rate to half the level of that of the wild type. Deletion of the C-terminal one (K230), two (S229 and K230), three (S229 to L231), or six amino acids (S229 to D234) as well as the insertion of five giycines between S229 and K230 or between S220 and Q221 did not affect significantly the cleavage while the deletion of 20 amino acids (Q218 to S237) decreased the processing rate to 73% of that of the wild type. These results rule out the possibility that the C-terminal region plays a role as a spacer in right placement of the NIa-NIb junction sequence and demonstrate that the C-terminal 20 amino acids from Q218 to S237 are not crucial for the cis-cleavage of the NIa-NIb junction sequence.
URI
https://scholarworks.unist.ac.kr/handle/201301/7132
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032006499
DOI
10.1006/viro.1997.8938
ISSN
0042-6822
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