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Ghim, Cheol-Min
Physical Biology & Biological Physics
Research Interests
  • Control of complex networks, Stochastic fluctuations in a cell, Biochemical information processing, Human microbiome, Ecological inference, Nonequilibrium active system

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Elucidating the role of metal ions in carbonic anhydrase catalysis

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Title
Elucidating the role of metal ions in carbonic anhydrase catalysis
Author
Kim, Jin KyunLee, CheolLim, Seon WooAndring, Jacob T.Adhikari, AniruddhaMcKenna, RobertGhim, Cheol-MinKim, Chae Un
Issue Date
2020-09
Publisher
Nature Publishing Group
Citation
NATURE COMMUNICATIONS, v.11, no.1, pp.4557
Abstract
Why metalloenzymes often show dramatic changes in their catalytic activity when subjected to chemically similar but non-native metal substitutions is a long-standing puzzle. Here, we report on the catalytic roles of metal ions in a model metalloenzyme system, human carbonic anhydrase II (CA II). Through a comparative study on the intermediate states of the zinc-bound native CA II and non-native metal-substituted CA IIs, we demonstrate that the characteristic metal ion coordination geometries (tetrahedral for Zn2+, tetrahedral to octahedral conversion for Co2+, octahedral for Ni2+, and trigonal bipyramidal for Cu2+) directly modulate the catalytic efficacy. In addition, we reveal that the metal ions have a long-range (~10 Å) electrostatic effect on restructuring water network in the active site. Our study provides evidence that the metal ions in metalloenzymes have a crucial impact on the catalytic mechanism beyond their primary chemical properties. © 2020, The Author(s).
URI
https://scholarworks.unist.ac.kr/handle/201301/48187
URL
https://www.nature.com/articles/s41467-020-18425-5
DOI
10.1038/s41467-020-18425-5
ISSN
2041-1723
Appears in Collections:
PHY_Journal Papers
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