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Ghim, Cheol-Min
Physical Biology & Biological Physics
Research Interests
  • Control of complex networks, Stochastic fluctuations in a cell, Biochemical information processing, Human microbiome, Ecological inference, Nonequilibrium active system


Elucidating the role of metal ions in carbonic anhydrase catalysis

DC Field Value Language Kim, Jin Kyun ko Lee, Cheol ko Lim, Seon Woo ko Andring, Jacob T. ko Adhikari, Aniruddha ko McKenna, Robert ko Ghim, Cheol-Min ko Kim, Chae Un ko 2020-10-22T08:04:16Z - 2020-09-23 ko 2020-09 ko
dc.identifier.citation NATURE COMMUNICATIONS, v.11, no.1, pp.4557 ko
dc.identifier.issn 2041-1723 ko
dc.identifier.uri -
dc.description.abstract Why metalloenzymes often show dramatic changes in their catalytic activity when subjected to chemically similar but non-native metal substitutions is a long-standing puzzle. Here, we report on the catalytic roles of metal ions in a model metalloenzyme system, human carbonic anhydrase II (CA II). Through a comparative study on the intermediate states of the zinc-bound native CA II and non-native metal-substituted CA IIs, we demonstrate that the characteristic metal ion coordination geometries (tetrahedral for Zn2+, tetrahedral to octahedral conversion for Co2+, octahedral for Ni2+, and trigonal bipyramidal for Cu2+) directly modulate the catalytic efficacy. In addition, we reveal that the metal ions have a long-range (~10 Å) electrostatic effect on restructuring water network in the active site. Our study provides evidence that the metal ions in metalloenzymes have a crucial impact on the catalytic mechanism beyond their primary chemical properties. © 2020, The Author(s). ko
dc.language 영어 ko
dc.publisher Nature Publishing Group ko
dc.title Elucidating the role of metal ions in carbonic anhydrase catalysis ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-85090797188 ko
dc.identifier.wosid 000607111200018 ko
dc.type.rims ART ko
dc.identifier.doi 10.1038/s41467-020-18425-5 ko
dc.identifier.url ko
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