The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmission
Cited 0 times inCited 0 times in
- The voltage-gated potassium channel Shaker promotes sleep via thermosensitive GABA transmission
- Kim, Ji-hyung; Ki, Yoonhee; Lee, Hoyeon; Hur, Moon Seong; Baik, Bukyung; Hur, Jin-Hoe; Nam, Dougu; Lim, Chunghun
- Issue Date
- NATURE PUBLISHING GROUP
- COMMUNICATIONS BIOLOGY, v.3, no.1, pp.174
- Genes and neural circuits coordinately regulate animal sleep. However, it remains elusive how these endogenous factors shape sleep upon environmental changes. Here, we demonstrate that Shaker (Sh)-expressing GABAergic neurons projecting onto dorsal fan-shaped body (dFSB) regulate temperature-adaptive sleep behaviors in Drosophila. Loss of Sh function suppressed sleep at low temperature whereas light and high temperature cooperatively gated Sh effects on sleep. Sh depletion in GABAergic neurons partially phenocopied Sh mutants. Furthermore, the ionotropic GABA receptor, Resistant to dieldrin (Rdl), in dFSB neurons acted downstream of Sh and antagonized its sleep-promoting effects. In fact, Rdl inhibited the intracellular cAMP signaling of constitutively active dopaminergic synapses onto dFSB at low temperature. High temperature silenced GABAergic synapses onto dFSB, thereby potentiating the wake-promoting dopamine transmission. We propose that temperature-dependent switching between these two synaptic transmission modalities may adaptively tune the neural property of dFSB neurons to temperature shifts and reorganize sleep architecture for animal fitness.
Ji-hyung Kim and Yoonhee Ki et al. show that low temperatures suppress sleep in Drosophila by increasing GABA transmission in Shaker-expressing GABAergic neurons projecting onto the dorsal fan-shaped body, while high temperatures potentiate dopamine-induced arousal by reducing GABA transmission. This study highlights a role for Shaker in sleep modulation via a temperature-dependent switch in GABA signaling.
- Appears in Collections:
- BIO_Journal Papers
- Files in This Item:
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.