Transcriptional Regulator TonEBP Mediates Oxidative Damages in Ischemic Kidney Injury
Cited 0 times inCited 0 times in
- Transcriptional Regulator TonEBP Mediates Oxidative Damages in Ischemic Kidney Injury
- Yoo, Eun Jin; Lim, Sun Woo; Kang, Hyun Je; Park, Hyun; Yoon, Sora; Nam, Dougu; Sanada, Satoru; Kwon, Mi Jin; Lee-Kwon, Whaseon; Choi, Soo Youn; Kwon, Hyug Moo
- Issue Date
- CELLS, v.8, no.10, pp.1284
- TonEBP (tonicity-responsive enhancer binding protein) is a transcriptional regulator whose expression is elevated in response to various forms of stress including hyperglycemia, inflammation, and hypoxia. Here we investigated the role of TonEBP in acute kidney injury (AKI) using a line of TonEBP haplo-deficient mice subjected to bilateral renal ischemia followed by reperfusion (I/R). In the TonEBP haplo-deficient animals, induction of TonEBP, oxidative stress, inflammation, cell death, and functional injury in the kidney in response to I/R were all reduced. Analyses of renal transcriptome revealed that genes in several cellular pathways including peroxisome and mitochondrial inner membrane were suppressed in response to I/R, and the suppression was relieved in the TonEBP deficiency. Production of reactive oxygen species (ROS) and the cellular injury was reproduced in a renal epithelial cell line in response to hypoxia, ATP depletion, or hydrogen peroxide. The knockdown of TonEBP reduced ROS production and cellular injury in correlation with increased expression of the suppressed genes. The cellular injury was also blocked by inhibitors of necrosis. These results demonstrate that ischemic insult suppresses many genes involved in cellular metabolism leading to local oxidative stress by way of TonEBP induction. Thus, TonEBP is a promising target to prevent AKI.
- Appears in Collections:
- SLS_Journal Papers
- Files in This Item:
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.