File Download

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

명경재

Myung, Kyungjae
Center for Genomic Integrity
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage

Author(s)
Lee, Nam SooChung, Hee JinKim, Hyoung-JuneLee, Seo YunJi, Jae-HoonSeo, YoojeongHan, Seung HunChoi, MinjiYun, MiyongLee, Seok-GeunMyung, KyungjaeKim, YonghwanKang, Ho ChulKim, Hongtae
Issued Date
2016-01
DOI
10.1038/ncomms10463
URI
https://scholarworks.unist.ac.kr/handle/201301/18282
Fulltext
http://www.nature.com/ncomms/2016/160119/ncomms10463/full/ncomms10463.html
Citation
NATURE COMMUNICATIONS, v.7, pp.10463
Abstract
RAP80 localizes to sites of DNA insults to enhance the DNA-damage responses. Here we identify TRAIP/RNF206 as a novel RAP80-interacting protein and find that TRAIP is necessary for translocation of RAP80 to DNA lesions. Depletion of TRAIP results in impaired accumulation of RAP80 and functional downstream partners, including BRCA1, at DNA lesions. Conversely, accumulation of TRAIP is normal in RAP80-depleted cells, implying that TRAIP acts upstream of RAP80 recruitment to DNA lesions. TRAIP localizes to sites of DNA damage and cells lacking TRAIP exhibit classical DNA-damage response-defect phenotypes. Biochemical analysis reveals that the N terminus of TRAIP is crucial for RAP80 interaction, while the C terminus of TRAIP is required for TRAIP localization to sites of DNA damage through a direct interaction with RNF20-RNF40. Taken together, our findings demonstrate that the novel RAP80-binding partner TRAIP regulates recruitment of the damage signalling machinery and promotes homologous recombination
Publisher
NATURE PUBLISHING GROUP
ISSN
2041-1723
Keyword
DOUBLE-STRAND BREAKSINTERACTING PROTEIN TRIPTUMOR-NECROSIS-FACTORKAPPA-B ACTIVATIONHOMOLOGOUS RECOMBINATIONH2B UBIQUITINATIONREPAIR PROTEINSTARGETS BRCA1HISTONE H2BTRANSCRIPTION

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.