File Download

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

강세병

Kang, Sebyung
Protein Nanobio Lab.
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Lumazine Synthase Protein Nanoparticle-Gd(III)-DOTA Conjugate as a T1 contrast agent for high-field MRI

Author(s)
Song, YoungKyuKang, Young JiJung, HoesuKim, HansolKang, SebyungCho, Hyungjoon
Issued Date
2015-10
DOI
10.1038/srep15656
URI
https://scholarworks.unist.ac.kr/handle/201301/17696
Fulltext
http://www.nature.com/articles/srep15656
Citation
SCIENTIFIC REPORTS, v.5, pp.15656
Abstract
With the applications of magnetic resonance imaging (MRI) at higher magnetic fields increasing, there is demand for MRI contrast agents with improved relaxivity at higher magnetic fields. Macromolecule-based contrast agents, such as protein-based ones, are known to yield significantly higher r(1) relaxivity at low fields, but tend to lose this merit when used as T-1 contrast agents (r(1)/r(2) = 0.5 similar to 1), with their r(1) decreasing and r(2) increasing as magnetic field strength increases. Here, we developed and characterized an in vivo applicable magnetic resonance (MR) positive contrast agent by conjugating Gd(III)-chelating agent complexes to lumazine synthase isolated from Aquifex aeolicus (AaLS). The r(1) relaxivity of Gd(III)-DOTA-AaLS-R108C was 16.49 mM(-1)s(-1) and its r(1)/r(2) ratio was 0.52 at the magnetic field strength of 7 T. The results of 3D MR angiography demonstrated the feasibility of vasculature imaging within 2 h of intravenous injection of the agent and a significant reduction in T-1 values were observed in the tumor region 7 h post-injection in the SCC-7 flank tumor model. Our findings suggest that Gd(III)-DOTA-AaLS-R108C could serve as a potential theranostic nanoplatform at high magnetic field strength.
Publisher
NATURE PUBLISHING GROUP
ISSN
2045-2322
Keyword
TRANSFER RADICAL POLYMERIZATIONTARGETED DRUG-DELIVERYCAGE NANOPARTICLESGADOLINIUM COMPLEXAQUIFEX-AEOLICUSVIRAL CAPSIDSMOSAIC-VIRUSCANCER-CELLSIRON-OXIDERELAXIVITY

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.