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Ko, Myunggon
Molecular Immunology & Cancer Epigenetics
Research Interests
  • TET proteins, DNA hydroxymethylation, oncogenesis, cancer stem cells, cancer therapy

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Expression of SRG3, a core component of mouse SWI/SNF chromatin-remodeling complex, is regulated by cooperative interactions between Sp1/Sp3 and Ets transcription factors

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Title
Expression of SRG3, a core component of mouse SWI/SNF chromatin-remodeling complex, is regulated by cooperative interactions between Sp1/Sp3 and Ets transcription factors
Author
Ahn, JeongeunKo, Myung GonLee, KyuyoungOh, JaehakJeon, SunghoSeong, Rho H
Issue Date
2005-12
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.338, no.3, pp.1435 - 1446
Abstract
SRG3, a mouse homolog of yeast SW13 and human BAF155, is known to be a core component of SWI/SNF chromatin-remodeling complex. We have previously shown that SRG3 plays essential roles in early mouse embryogenesis, brain development, and T-cell development. SRG3 gene expression was differentially regulated depending on the developmental stages and exhibited tissue-specific pattern. In this study, we showed that the functional interactions between Sp and Ets family transcription factors are crucial for the SRG3 expression. Sp I and Sp3 specifically bound to the two canonical Sp-binding sites (GC boxes) at -152 and -114, and a non-canonical Sp-binding site (CCTCCT inotif) at -108 in the SRG3 promoter. Using Drosophila SL2 cells, we found that Various Sp or Ets family members activate the SRG3 promoter through these Sp- or Ets-binding sites, respectively, in a dose-dependent manner. Intriguingly, different combinatorial expression of Ets and Sp factors in SL2 cells resulted in either strong synergistic activation or repression of the SRG3 promoter activity. Moreover, the Sp-mediated activation of SRG3 promoter required the intact Ets-binding element. Taken together, these results Suggest that diverse interactions between Sp1/Sp3 and Ets factors are crucial for the SRG3 gene expression. (c) 2005 Elsevier Inc. All rights reserved.
URI
https://scholarworks.unist.ac.kr/handle/201301/12653
URL
http://www.sciencedirect.com/science/article/pii/S0006291X05023594#
DOI
10.1016/j.bbrc.2005.10.107
ISSN
0006-291X
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BIO_Journal Papers
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