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- Rhee, Hyun-Woo
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An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier
- Author(s)
- Lee, Jisu, Song, Eun Kyung, Bae, Yoonji, Min, Junseon, Rhee, Hyun-Woo, Park, Tae Joo, Kim, Moonil, Kang, Sebyung
- Issued Date
- 2015-07
- Citation
- CHEMICAL COMMUNICATIONS, v.51, no.54, pp.10945 - 10948
- Abstract
- A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2-ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.
- Publisher
- ROYAL SOC CHEMISTRY
- ISSN
- 1359-7345
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