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Park, Tae Joo
Developmental Morphogenesis Lab
Research Interests
  • Morphogenesis, chondrogenesis, ciliogenesis

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An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier

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Title
An enhanced ascorbate peroxidase 2/antibody-binding domain fusion protein (APEX2-ABD) as a recombinant target-specific signal amplifier
Author
Lee, JisuSong, Eun KyungBae, YoonjiMin, JunseonRhee, Hyun-WooPark, Tae JooKim, MoonilKang, Sebyung
Issue Date
2015-07
Publisher
ROYAL SOC CHEMISTRY
Citation
CHEMICAL COMMUNICATIONS, v.51, no.54, pp.10945 - 10948
Abstract
A recombinant target-specific signal amplifier was constructed by genetically fusing the enhanced ascorbate peroxidase 2 (APEX2) and an antibody-binding domain (ABD). The fusion protein APEX2-ABD possessed the peroxidase activity and the antibody-binding capability simultaneously and replaced the conventional HRP-conjugated secondary antibodies in a TSA assay for amplifying fluorescence signals.
URI
https://scholarworks.unist.ac.kr/handle/201301/12042
URL
http://pubs.rsc.org/en/Content/ArticleLanding/2015/CC/c5cc02409a#!divAbstract
DOI
10.1039/C5CC02409A
ISSN
1359-7345
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