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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Collapsin response mediator protein-2 regulates neurite formation by modulating tubulin GTPase activity

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Title
Collapsin response mediator protein-2 regulates neurite formation by modulating tubulin GTPase activity
Author
Chae, Young ChanLee, SukmookHeo, KyunHa, Sang HoonJung, YonwooKim, Jong HyunIhara, YasuoSuh, Pann-GhillRyu, Sung Ho
Issue Date
2009-12
Publisher
ELSEVIER SCIENCE INC
Citation
CELLULAR SIGNALLING, v.21, no.12, pp.1818 - 1826
Abstract
Collapsin response mediator protein-2 (CRMP-2) plays a key role in axonal development by regulating microtubule dynamics. However, the molecular mechanisms underlying this function have not been clearly elucidated. In this study, we demonstrated that hCRMP-2, specifically amino acid residues 480-509, is essential for stimulating tubulin GTPase activity. We also found that the GTPase-activating protein (GAP) activity of hCRMP-2 was important for microtubule assembly and neurite formation in differentiated PC12 pheochromocytoma cell lines. Mutant hCRMP-2, lacking arginine residues responsible for GAP activity, inhibited microtubule assembly and neurite formation. Interestingly, we found that the N-terminal region (amino acids 150-299) of hCRMP-2 had an inhibitory role on GAP activity via a direct interaction with the C-terminal region (amino acids 480-509). Our results suggest that CRMP-2 as a tubulin direct binder may be a GAP of tubulin in neurite formation and that its CAP activity may be regulated by an intramolecular interaction with an N-terminal inhibitory region.
URI
https://scholarworks.unist.ac.kr/handle/201301/10095
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=70349784226
DOI
10.1016/j.cellsig.2009.07.017
ISSN
0898-6568
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BIO_Journal Papers
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