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- Kim, Hajin
- Single Molecule Biophysics Lab.
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An improved surface passivation method for single-molecule studies
- Author(s)
- Hua, Boyang, Han, Kyu Young, Zhou, Ruobo, Kim, Hajin, Shi, Xinghua, Abeysirigunawardena, Sanjaya C., Jain, Ankur, Singh, Digvijay, Aggarwal, Vasudha, Woodson, Sarah A., Ha, Taek Jip
- Issued Date
- 2014-12
- Citation
- NATURE METHODS, v.11, no.12, pp.1233 - 1236
- Abstract
- We report a surface passivation method based on dichlorodimethylsilane (DDS)-Tween-20 for in vitro single-molecule studies, which, under the conditions tested here, more efficiently prevented nonspecific binding of biomolecules than the standard poly (ethylene glycol) surface. The DDS-Tween-20 surface was simple and inexpensive to prepare and did not perturb the behavior and activities of tethered biomolecules. It can also be used for single-molecule imaging in the presence of high concentrations of labeled species in solution.
- Publisher
- NATURE PUBLISHING GROUP
- ISSN
- 1548-7091
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