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Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Laboratory
Research Interests
  • Biofuels production
  • Gene discovery
  • Protein engineering
  • Development of gene expression systems
  • Metabolic pathway regulation
  • Metabolic pathway optimization using functional genomics
  • Synthetic biology
  • Development of biosensors

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A Salmonella-based, propionate-inducible, expression system for Salmonella enterica

Cited 7 times inthomson ciCited 8 times inthomson ci
Title
A Salmonella-based, propionate-inducible, expression system for Salmonella enterica
Author
Lee, Sung KukKeasling, Jay D.
Keywords
Expression system; Inducible promoter; prpBCDE promoter; prpR; Salmonella enterica
Issue Date
2006-08
Publisher
ELSEVIER SCIENCE BV
Citation
GENE, v.377, no., pp.6 - 11
Abstract
The expression and regulatory properties of a propionate-regulated overexpression system (Salmonella enterica prpBCDE promoter (PprpB) and positive regulator (prpR)) were evaluated in wild-type S. enterica serovar Typhimurium TR6583 and prpB- or prpD- versions of this strain and compared with the arabinose-regulated T7 expression system. The wild-type strain showed low expression in the absence of propionate and high expression in the presence of propionate under all growth conditions. In 96-well plates and culture tubes, the wild-type strain exhibited a long delay before full induction; the time delay was significantly shorter in shake flasks. The prpD- strain exhibited low expression in the presence of glucose, highly regulatable expression over a wide range of propionate concentrations, and, in contrast to the wild-type strain, fast induction to full expression under all growth conditions. In contrast, the prpB- strain showed very high background expression in both culture tubes and shake flasks.
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DOI
10.1016/j.gene.2006.02.013
ISSN
0378-1119
Appears in Collections:
ECHE_Journal Papers
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