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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 11 | - |
dc.citation.startPage | 6 | - |
dc.citation.title | GENE | - |
dc.citation.volume | 377 | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.contributor.author | Keasling, Jay D. | - |
dc.date.accessioned | 2023-12-22T09:43:45Z | - |
dc.date.available | 2023-12-22T09:43:45Z | - |
dc.date.created | 2014-11-18 | - |
dc.date.issued | 2006-08 | - |
dc.description.abstract | The expression and regulatory properties of a propionate-regulated overexpression system (Salmonella enterica prpBCDE promoter (PprpB) and positive regulator (prpR)) were evaluated in wild-type S. enterica serovar Typhimurium TR6583 and prpB- or prpD- versions of this strain and compared with the arabinose-regulated T7 expression system. The wild-type strain showed low expression in the absence of propionate and high expression in the presence of propionate under all growth conditions. In 96-well plates and culture tubes, the wild-type strain exhibited a long delay before full induction; the time delay was significantly shorter in shake flasks. The prpD- strain exhibited low expression in the presence of glucose, highly regulatable expression over a wide range of propionate concentrations, and, in contrast to the wild-type strain, fast induction to full expression under all growth conditions. In contrast, the prpB- strain showed very high background expression in both culture tubes and shake flasks. | - |
dc.identifier.bibliographicCitation | GENE, v.377, pp.6 - 11 | - |
dc.identifier.doi | 10.1016/j.gene.2006.02.013 | - |
dc.identifier.issn | 0378-1119 | - |
dc.identifier.scopusid | 2-s2.0-33745958420 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/9105 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33745958420 | - |
dc.identifier.wosid | 000239669300002 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | A Salmonella-based, propionate-inducible, expression system for Salmonella enterica | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | expression system | - |
dc.subject.keywordAuthor | inducible promoter | - |
dc.subject.keywordAuthor | prpBCDE promoter | - |
dc.subject.keywordAuthor | prpR | - |
dc.subject.keywordAuthor | Salmonella enterica | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | TYPHIMURIUM LT2 | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | ARABAD PROMOTER | - |
dc.subject.keywordPlus | PRP LOCUS |
- |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | OPERON | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | 2-METHYLCITRATE | - |
dc.subject.keywordPlus | CATABOLISM | - |
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