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Kim, Taesung
Microfluidics & Nanomechatronics Lab (μFNM)
Research Interests
  • Micro-/Nanofluidics

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Chemostat-like microfluidic platform for highly sensitive detection of heavy metal ions using microbial biosensors

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Title
Chemostat-like microfluidic platform for highly sensitive detection of heavy metal ions using microbial biosensors
Author
Kim, MinseokLim, Ji WonKim, Hyun JuLee, Sung KukLee, Sang JunKim, Taesung
Issue Date
2015-03
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Citation
BIOSENSORS & BIOELECTRONICS, v.65, pp.257 - 264
Abstract
Reporter-gene-based microbial biosensors have high potential for detecting small molecules, including heavy metal ions (HMIs), in a sensitive and selective manner by involving low costs. However, the sensitivity and dynamic range of the sensing mechanism are largely limited by the conventional culture environment that relies on the batch-type addition of the small molecules in nutrients and the subsequent genetic induction of sensing microbes. Here, we describe a high-throughput, chemostat-like microfluidic platform that can continuously supply both nutrients and inducers (HMIs) using microfabricated ratchet structures and a mixing microchannel network. We found that the microfluidic platform not only allowed microbial biosensors to be highly concentrated in a detection microchamber array but also enabled them to continuously grow and control synthetic genetic circuits in response to heavy metals. We also demonstrated that the combination of the platform and microbial biosensors enhanced the sensitivity for detecting divalent lead and cadmium ions by approximately three orders of magnitude relative to conventional batch-type methods. Because the platform is portable and only requires small sample volumes and fluorescent detection, the chemostat-like microfluidic platform in conjunction with microbial biosensors could be widely utilized to facilitate the specific and sensitive detection of molecular analytes on a chip.
URI
https://scholarworks.unist.ac.kr/handle/201301/9098
URL
http://www.sciencedirect.com/science/article/pii/S0956566314008264
DOI
10.1016/j.bios.2014.10.028
ISSN
0956-5663
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MNE_Journal Papers
ECHE_Journal Papers
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