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Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Lab.
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Chemostat-like microfluidic platform for highly sensitive detection of heavy metal ions using microbial biosensors

Author(s)
Kim, MinseokLim, Ji WonKim, Hyun JuLee, Sung KukLee, Sang JunKim, Taesung
Issued Date
2015-03
DOI
10.1016/j.bios.2014.10.028
URI
https://scholarworks.unist.ac.kr/handle/201301/9098
Fulltext
http://www.sciencedirect.com/science/article/pii/S0956566314008264
Citation
BIOSENSORS & BIOELECTRONICS, v.65, pp.257 - 264
Abstract
Reporter-gene-based microbial biosensors have high potential for detecting small molecules, including heavy metal ions (HMIs), in a sensitive and selective manner by involving low costs. However, the sensitivity and dynamic range of the sensing mechanism are largely limited by the conventional culture environment that relies on the batch-type addition of the small molecules in nutrients and the subsequent genetic induction of sensing microbes. Here, we describe a high-throughput, chemostat-like microfluidic platform that can continuously supply both nutrients and inducers (HMIs) using microfabricated ratchet structures and a mixing microchannel network. We found that the microfluidic platform not only allowed microbial biosensors to be highly concentrated in a detection microchamber array but also enabled them to continuously grow and control synthetic genetic circuits in response to heavy metals. We also demonstrated that the combination of the platform and microbial biosensors enhanced the sensitivity for detecting divalent lead and cadmium ions by approximately three orders of magnitude relative to conventional batch-type methods. Because the platform is portable and only requires small sample volumes and fluorescent detection, the chemostat-like microfluidic platform in conjunction with microbial biosensors could be widely utilized to facilitate the specific and sensitive detection of molecular analytes on a chip.
Publisher
ELSEVIER ADVANCED TECHNOLOGY
ISSN
0956-5663
Keyword (Author)
ChemostatGene expressionHeavy metal ionsMicrobial biosensorMicrofluidic platform
Keyword
WHOLE-CELLENGINEERED BACTERIACADMIUMDIFFUSIONTOXICITYPROTEINSPECTROMETRYPOLLUTIONSYSTEMSYEAST

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