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Kim, Taesung
Microfluidics & Nanomechatronics Lab (μFNM)
Research Interests
  • Microfluidics & Nanofluidics
  • Nanoscale Transport Phenomena
  • MEMS & BioMEMS
  • Nanofabrication & Nanomechatronics


Chemostat-like microfluidic platform for highly sensitive detection of heavy metal ions using microbial biosensors

DC Field Value Language Kim, Minseok ko Lim, Ji Won ko Kim, Hyun Ju ko Lee, Sung Kuk ko Lee, Sang Jun ko Kim, Taesung ko 2014-11-21T00:12:47Z - 2014-11-18 ko 2015-03 ko
dc.identifier.citation BIOSENSORS & BIOELECTRONICS, v.65, pp.257 - 264 ko
dc.identifier.issn 0956-5663 ko
dc.identifier.uri -
dc.description.abstract Reporter-gene-based microbial biosensors have high potential for detecting small molecules, including heavy metal ions (HMIs), in a sensitive and selective manner by involving low costs. However, the sensitivity and dynamic range of the sensing mechanism are largely limited by the conventional culture environment that relies on the batch-type addition of the small molecules in nutrients and the subsequent genetic induction of sensing microbes. Here, we describe a high-throughput, chemostat-like microfluidic platform that can continuously supply both nutrients and inducers (HMIs) using microfabricated ratchet structures and a mixing microchannel network. We found that the microfluidic platform not only allowed microbial biosensors to be highly concentrated in a detection microchamber array but also enabled them to continuously grow and control synthetic genetic circuits in response to heavy metals. We also demonstrated that the combination of the platform and microbial biosensors enhanced the sensitivity for detecting divalent lead and cadmium ions by approximately three orders of magnitude relative to conventional batch-type methods. Because the platform is portable and only requires small sample volumes and fluorescent detection, the chemostat-like microfluidic platform in conjunction with microbial biosensors could be widely utilized to facilitate the specific and sensitive detection of molecular analytes on a chip. ko
dc.description.statementofresponsibility close -
dc.language 영어 ko
dc.title Chemostat-like microfluidic platform for highly sensitive detection of heavy metal ions using microbial biosensors ko
dc.type ARTICLE ko
dc.identifier.scopusid 2-s2.0-84908644897 ko
dc.identifier.wosid 000348880000038 ko
dc.type.rims ART ko
dc.description.wostc 0 *
dc.description.scopustc 0 * 2015-05-06 * 2014-11-18 *
dc.identifier.doi 10.1016/j.bios.2014.10.028 ko
dc.identifier.url ko
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