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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Purification and Characterization of a New Galactoside Specific Lectin from Trichosanthes Kirlowii Root

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Title
Purification and Characterization of a New Galactoside Specific Lectin from Trichosanthes Kirlowii Root
Author
Yun, Doo HeePark, Eun JuPark, Jong OkLee, Young HanSeo, Jeong KonRyu, Sung HoSuh, Pann-GhillKim, Hee Sook
Issue Date
1995-01
Publisher
KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY
Citation
BMB REPORTS, v.28, no.1, pp.6 - 11
Abstract
A new lectin, named Tf~A, was purified from Trichosanthes kirilowii root by acid-treated Sepharose 6B, Mono-Q, and TSK-gel 3000SW column sequential chromatography. The lectin appeared homogeneous by native gel electrophoresis at pH 4.3 and gave two protein bands of Mr=31 and 28 kDa by SDS-PAGE. The N-terminal amino acid sequences of the polypeptides of TRA have not been reported in amino acid sequences of the lectins. TRA lectin fomoed a precipitate with asialofetuin, neuraminidase-treated fetuin. A sugar inhibition assay indicated that N-acetyl-D-galactosamine, among the monosaccharides tested, was the most potent inhibitor of TRA-induced hemagglutination. Asialofetuin showed a 260-times stronger inhibitory activity than N-acetyl-D-galactosamine. TRA lectin also showed agglutination with normal leukocytes and lymphoma cells, but not with premature hemopoietic cells. These results suggest that TRA is a novel plant lectin.
URI
https://scholarworks.unist.ac.kr/handle/201301/9061
URL
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=E1MBB7_1995_v28n1_6
ISSN
1976-6696
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