Purification and Characterization of a New Galactoside Specific Lectin from Trichosanthes Kirlowii Root
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yun, Doo Hee | ko |
dc.contributor.author | Park, Eun Ju | ko |
dc.contributor.author | Park, Jong Ok | ko |
dc.contributor.author | Lee, Young Han | ko |
dc.contributor.author | Seo, Jeong Kon | ko |
dc.contributor.author | Ryu, Sung Ho | ko |
dc.contributor.author | Suh, Pann-Ghill | ko |
dc.contributor.author | Kim, Hee Sook | ko |
dc.date.available | 2014-11-21T00:07:37Z | - |
dc.date.created | 2014-11-20 | ko |
dc.date.issued | 1995-01 | ko |
dc.identifier.citation | BMB REPORTS, v.28, no.1, pp.6 - 11 | ko |
dc.identifier.issn | 1976-6696 | ko |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/9061 | - |
dc.description.abstract | A new lectin, named Tf~A, was purified from Trichosanthes kirilowii root by acid-treated Sepharose 6B, Mono-Q, and TSK-gel 3000SW column sequential chromatography. The lectin appeared homogeneous by native gel electrophoresis at pH 4.3 and gave two protein bands of Mr=31 and 28 kDa by SDS-PAGE. The N-terminal amino acid sequences of the polypeptides of TRA have not been reported in amino acid sequences of the lectins. TRA lectin fomoed a precipitate with asialofetuin, neuraminidase-treated fetuin. A sugar inhibition assay indicated that N-acetyl-D-galactosamine, among the monosaccharides tested, was the most potent inhibitor of TRA-induced hemagglutination. Asialofetuin showed a 260-times stronger inhibitory activity than N-acetyl-D-galactosamine. TRA lectin also showed agglutination with normal leukocytes and lymphoma cells, but not with premature hemopoietic cells. These results suggest that TRA is a novel plant lectin. | ko |
dc.description.statementofresponsibility | close | - |
dc.language | 영어 | ko |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | ko |
dc.title | Purification and Characterization of a New Galactoside Specific Lectin from Trichosanthes Kirlowii Root | ko |
dc.type | ARTICLE | ko |
dc.type.rims | ART | ko |
dc.identifier.url | http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=E1MBB7_1995_v28n1_6 | ko |
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