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Kwon, Hyug Moo
Immunometabolism and Cancer Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorder, Genomic instability

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Size selected mRNA induces expression of P-aminohippurate transport in Xenopus oocytes

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Title
Size selected mRNA induces expression of P-aminohippurate transport in Xenopus oocytes
Author
Kwon, O.Kwon, H. MooHong, S.K.Goldinger, J.M.
Issue Date
1989
Publisher
Society for Experimental Biology and Medicine
Citation
PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE. SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE (NEW YORK, N. Y.), v.192, no.2, pp.205 - 208
Abstract
Xenopus oocytes were injected with size-fractionated mRNA isolated from the renal cortex of rabbit kidney and after 4 days incubation, PAH uptake in oocytes injected with mRNA (0.7-1.3 kb) was 8 to 45 fold that of the water injected controls. The oocyte to medium ratio of accumulated PAH was 1.95. The Km and Vmax for transport were 333 μM and 66.6 nmoles·oocyte-1·min-1, respectively. This Km is similar to that reported for PAH transport in intact kidneys and slices. The uptake of PAH was unaffected by the absence of Na+ or the presence of probenecid. Expression of the transport represents the first step in an effort to clone and identify the gene for PAH transport.
URI
https://scholarworks.unist.ac.kr/handle/201301/8720
ISSN
0037-9727
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