Size selected mRNA induces expression of P-aminohippurate transport in Xenopus oocytes
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- Size selected mRNA induces expression of P-aminohippurate transport in Xenopus oocytes
- Kwon, O.; Kwon, H. Moo; Hong, S.K.; Goldinger, J.M.
- Issue Date
- Society for Experimental Biology and Medicine
- PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE. SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE (NEW YORK, N. Y.), v.192, no.2, pp.205 - 208
- Xenopus oocytes were injected with size-fractionated mRNA isolated from the renal cortex of rabbit kidney and after 4 days incubation, PAH uptake in oocytes injected with mRNA (0.7-1.3 kb) was 8 to 45 fold that of the water injected controls. The oocyte to medium ratio of accumulated PAH was 1.95. The Km and Vmax for transport were 333 μM and 66.6 nmoles·oocyte-1·min-1, respectively. This Km is similar to that reported for PAH transport in intact kidneys and slices. The uptake of PAH was unaffected by the absence of Na+ or the presence of probenecid. Expression of the transport represents the first step in an effort to clone and identify the gene for PAH transport.
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