BROWSE

Related Researcher

Author's Photo

Jang, Jaesung
Sensors & Aerosols Lab
Research Interests
  • Bio-MEMS
  • Bioaerosols
  • Sensor
  • Aerosol Cleaning
  • Fluid Mechanics at microscales

ITEM VIEW & DOWNLOAD

Electrical immunosensor based on dielectrophoretically-deposited carbon nanotubes for detection of influenza virus H1N1

Cited 1 times inthomson ciCited 0 times inthomson ci
Title
Electrical immunosensor based on dielectrophoretically-deposited carbon nanotubes for detection of influenza virus H1N1
Author
Singh, RenuSharma, AbhinavHong, SeongkyeolJang, Jaesung
Issue Date
2014-11
Publisher
ROYAL SOC CHEMISTRY
Citation
ANALYST, v.139, pp.5415 - 5421
Abstract
The influenza virus has received extensive attention due to the recent H1N1 pandemics originating from swine. This study reports a label-free, highly sensitive, and selective electrical immunosensor for the detection of influenza virus H1N1 based on dielectrophoretically deposited single-walled carbon nanotubes (SWCNTs). COOH-functionalized SWCNTs were deposited on a self-assembled monolayer of polyelectrolyte polydiallyldimethyl-ammonium chloride (PDDA) between two gold electrodes by dielectrophoretic and electrostatic forces, which resulted in reproducible, uniform, aligned, and aggregation-free SWCNT channels (2-10 μm in length). Avidin was immobilized onto the PDDA-SWCNT channels, and viral antibodies were immobilized using biotin-avidin coupling. The resistance of the channels increased with the binding of the influenza viruses to the antibodies. These immunosensors showed linear behavior as the virus concentration was varied from 1 to 104 PFU ml−1 along with a detection time of 30 min. The immunosensors with a 2 μm channel length detected 1 PFU ml−1 of the influenza virus accurately (R2 = 0.99) and selectively from MS2 bacteriophages. These immunosensors have the potential to become an important component of a point-of-care test kit that will enable a rapid clinical diagnosis.
URI
https://scholarworks.unist.ac.kr/handle/201301/8681
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84908321166
DOI
10.1039/c4an01335b
ISSN
0003-2654
Appears in Collections:
MEN_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU