TRAP1 functions in the morphogenesis of the embryonic kidney

Abstract

TNF receptor-associated protein1 (TRAP1) is a mitochondrial molecular chaperon with highhomology with a cytosolic chaperon HSP90. It has been shown that TRAP1 functions as aninhibitor for apoptosis by preventing cytochrome-c release from mitochondria. In addition,TRAP1 seems to play critical roles in metabolic processes for energy production, such asglycolysis and β-oxidation. It has also been reported that TRAP1 is a direct target of PTEN-induced kinase 1 (PINK1) and may be a cause of Parkinson’s disease (PD) in humans. Althoughthe biochemical functions of TRAP1 are under intense study for the physiology and treatmentof various cancers, its roles in vertebrate development have not been reported. This studyshows that Xenopus TRAP1 is strongly expressed in the developing muscle, kidney, and braintissues. Perturbation of TRAP1 function by treating TRAP1 inhibiter GTPP or microinjection ofantisense-morpholino oligo (MO) caused mild defects in striated muscle fiber formation.Furthermore, the looping patterns of developing kidney tubules were perturbed, indicating thatTRAP1 function is necessary for proper kidney development.