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Lim, Mi Hee
MetalloNeuroChemistry Lab (MNCL)
Research Interests
  • Neurodegenerative disease, small molecule design, network between metal, proteins, and ROS

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Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells

Cited 11 times inthomson ciCited 12 times inthomson ci
Title
Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells
Author
Lim, Mi Hee
Keywords
NOBEL LECTURE
Issue Date
2007
Publisher
NATURE PUBLISHING GROUP
Citation
NATURE PROTOCOLS, v.2, no.2, pp.408 - 415
Abstract
A procedure for the preparation of a copper(II) complex (CuFL) as a fluorescent nitric oxide ( NO) detector is described. The fluorescein-based ligand FL can be synthesized in seven reaction steps (overall yield similar to 20%), typically requiring a total time of 9 days. The CuFL probe allows for the detection of NO produced in mammalian cultured cells. The detailed protocol for the use of CuFL for imaging NO in human neuroblastoma SK-N-SH cells takes a total time of similar to 26 h. This includes plating cells on six-well tissue culture plates or imaging dishes, treatment with CuFL, stimulation of NO synthases and imaging by fluorescence microscopy.
URI
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DOI
10.1038/nprot.2007.43
ISSN
1754-2189
Appears in Collections:
PHY_Journal Papers
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