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| DC Field | Value | Language |
|---|---|---|
| dc.citation.endPage | 415 | - |
| dc.citation.number | 2 | - |
| dc.citation.startPage | 408 | - |
| dc.citation.title | NATURE PROTOCOLS | - |
| dc.citation.volume | 2 | - |
| dc.contributor.author | Lim, Mi Hee | - |
| dc.date.accessioned | 2023-12-22T09:38:00Z | - |
| dc.date.available | 2023-12-22T09:38:00Z | - |
| dc.date.created | 2014-11-11 | - |
| dc.date.issued | 2007 | - |
| dc.description.abstract | A procedure for the preparation of a copper(II) complex (CuFL) as a fluorescent nitric oxide ( NO) detector is described. The fluorescein-based ligand FL can be synthesized in seven reaction steps (overall yield similar to 20%), typically requiring a total time of 9 days. The CuFL probe allows for the detection of NO produced in mammalian cultured cells. The detailed protocol for the use of CuFL for imaging NO in human neuroblastoma SK-N-SH cells takes a total time of similar to 26 h. This includes plating cells on six-well tissue culture plates or imaging dishes, treatment with CuFL, stimulation of NO synthases and imaging by fluorescence microscopy. | - |
| dc.identifier.bibliographicCitation | NATURE PROTOCOLS, v.2, no.2, pp.408 - 415 | - |
| dc.identifier.doi | 10.1038/nprot.2007.43 | - |
| dc.identifier.issn | 1754-2189 | - |
| dc.identifier.scopusid | 2-s2.0-34347250850 | - |
| dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/8641 | - |
| dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34347250850 | - |
| dc.identifier.wosid | 000253138100022 | - |
| dc.language | 영어 | - |
| dc.publisher | NATURE PUBLISHING GROUP | - |
| dc.title | Preparation of a copper-based fluorescent probe for nitric oxide and its use in mammalian cultured cells | - |
| dc.type | Article | - |
| dc.description.journalRegisteredClass | scie | - |
| dc.description.journalRegisteredClass | scopus | - |
| dc.subject.keywordPlus | NOBEL LECTURE | - |
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