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Lee, Changsoo
Applied Biotechnology Lab for Environment (ABLE)
Research Interests
  • Bioconversion, Bioenergy, Biogas, Biological treatment, Biorefinery, Microbial community

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Use of order-specific primers to investigate the methanogenic diversity in acetate enrichment system

Cited 12 times inthomson ciCited 11 times inthomson ci
Title
Use of order-specific primers to investigate the methanogenic diversity in acetate enrichment system
Author
Shin, Seung GuLee, ChangsooHwang, KwanghyunAhn, Johng-HwaHwang, Seokhwan
Keywords
Denaturing gradient gel electrophoresis; Diversity of methanogens; Order-specific primers; Real-time quantitative PCR; Taxonomic level
Issue Date
2008-11
Publisher
SPRINGER HEIDELBERG
Citation
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.35, no.11, pp.1345 - 1352
Abstract
The applicability of order-specific primers in minimizing the possible underestimation of microbial diversity was evaluated via denaturing gradient gel electrophoresis (DGGE) analysis of a lab-scale anaerobic digester. Initially, a population analysis with real-time quantitative PCR demonstrated the existence of three methanogenic orders-Methanobacteriales, Methanomicrobiales, and Methanosarcinales-throughout the reaction period. DGGE analyses with three pairs of order-specific primers yielded eight operational taxonomic units (OTUs), whereas DGGE analysis with two independent Archaea-specific primers identified only five. Moreover, the order-specific primers amplified at least one OTU affiliated with each order, whereas no members of Methanobacteriales or Methanomicrobiales were identified with Archaea-specific primers in most samples. These findings provide evidence that order-specific analysis can detect the diversity of methanogens in greater detail than conventional Archaea-specific analysis.
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DOI
10.1007/s10295-008-0417-7
ISSN
1367-5435
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UEE_Journal Papers
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