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DC Field | Value | Language |
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dc.citation.endPage | 1352 | - |
dc.citation.number | 11 | - |
dc.citation.startPage | 1345 | - |
dc.citation.title | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.citation.volume | 35 | - |
dc.contributor.author | Shin, Seung Gu | - |
dc.contributor.author | Lee, Changsoo | - |
dc.contributor.author | Hwang, Kwanghyun | - |
dc.contributor.author | Ahn, Johng-Hwa | - |
dc.contributor.author | Hwang, Seokhwan | - |
dc.date.accessioned | 2023-12-22T08:36:04Z | - |
dc.date.available | 2023-12-22T08:36:04Z | - |
dc.date.created | 2014-11-05 | - |
dc.date.issued | 2008-11 | - |
dc.description.abstract | The applicability of order-specific primers in minimizing the possible underestimation of microbial diversity was evaluated via denaturing gradient gel electrophoresis (DGGE) analysis of a lab-scale anaerobic digester. Initially, a population analysis with real-time quantitative PCR demonstrated the existence of three methanogenic orders-Methanobacteriales, Methanomicrobiales, and Methanosarcinales-throughout the reaction period. DGGE analyses with three pairs of order-specific primers yielded eight operational taxonomic units (OTUs), whereas DGGE analysis with two independent Archaea-specific primers identified only five. Moreover, the order-specific primers amplified at least one OTU affiliated with each order, whereas no members of Methanobacteriales or Methanomicrobiales were identified with Archaea-specific primers in most samples. These findings provide evidence that order-specific analysis can detect the diversity of methanogens in greater detail than conventional Archaea-specific analysis. | - |
dc.identifier.bibliographicCitation | JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.35, no.11, pp.1345 - 1352 | - |
dc.identifier.doi | 10.1007/s10295-008-0417-7 | - |
dc.identifier.issn | 1367-5435 | - |
dc.identifier.scopusid | 2-s2.0-54049126447 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/8283 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=54049126447 | - |
dc.identifier.wosid | 000260009200021 | - |
dc.language | 영어 | - |
dc.publisher | SPRINGER HEIDELBERG | - |
dc.title | Use of order-specific primers to investigate the methanogenic diversity in acetate enrichment system | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Diversity of methanogens | - |
dc.subject.keywordAuthor | Taxonomic level | - |
dc.subject.keywordAuthor | Order-specific primers | - |
dc.subject.keywordAuthor | Real-time quantitative PCR | - |
dc.subject.keywordAuthor | Denaturing gradient gel electrophoresis | - |
dc.subject.keywordPlus | POLYMERASE-CHAIN-REACTION | - |
dc.subject.keywordPlus | 16S RIBOSOMAL-RNA | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | GROUP-SPECIFIC QUANTIFICATION | - |
dc.subject.keywordPlus | GRADIENT GEL-ELECTROPHORESIS | - |
dc.subject.keywordPlus | MICROBIAL COMMUNITY |
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dc.subject.keywordPlus | START-UP | - |
dc.subject.keywordPlus | ANAEROBIC BIOREACTOR | - |
dc.subject.keywordPlus | POPULATION-DYNAMICS | - |
dc.subject.keywordPlus | PROBE SETS |
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