File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher


Kang, Hyun-Wook
3D Biofabrication Lab.
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

A long-term storable gel-laden chip composite built in a multi-well plate enabling in situ cell encapsulation for high-throughput liver model

Kim, Min KyeongPark, JubinTak, SunghoPaek, KyurimBang, GeulWoo, Sang-MiRavichandran, Naresh KumarHong, Won GiKang, Hyun-WookKim, HyangBae, Ji YongKim, Jeong Ah
Issued Date
BIOFABRICATION, v.16, no.2, pp.025020
Hydrogels are widely used as scaffold materials for constructing in vitro three-dimensional microphysiological systems. However, their high sensitivity to various external cues hinders the development of hydrogel-laden, microscale, and high-throughput chips. Here, we have developed a long-term storable gel-laden chip composite built in a multi-well plate, which enables in situ cell encapsulation and facilitates high-throughput analysis. Through optimized chemical crosslinking and freeze-drying method (C/FD), we have achieved a high-quality of gel-laden chip composite with excellent transparency, uniform porosity, and appropriate swelling and mechanical characteristics. Besides collagen, decellularized extracellular matrix with tissue-specific biochemical compound has been applied as chip composite. As a ready-to-use platform, in situ cell encapsulation within the gel has been achieved through capillary force generated during gel reswelling. The liver-mimetic chip composite, comprising HepG2 cells or primary hepatocytes, has demonstrated favorable hepatic functionality and high sensitivity in drug testing. The developed fabrication process with improved stability of gels and storability allows chip composites to be stored at a wide range of temperatures for up to 28 d without any deformation, demonstrating off-the-shelf products. Consequently, this provides an exceptionally simple and long-term storable platform that can be utilized for an efficient tissue-specific modeling and various biomedical applications.
IOP Publishing
Keyword (Author)
freeze-dried hydrogeldecellularized extracellular matrixhigh-throughput analysisliver modeldrug assay


Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.