2D IR Study of the Aggregation Pathway of hIAPP oligomers

Abstract

Type 2 diabetes (T2D) is a disease in which human islet amyloid polypeptide (hIAPP) amyloid deposits attack β-cells, causing insulin production problems and the loss of blood sugar control. Although the formation process of hIAPP deposits has been studied for several decades, the early stage of the aggregation mechanism is not fully understood due to the transient nature of intermediate oligomers. In this regard, we slowed down the aggregation process by lowering the sample temperature and observed the aggregation process in detail. The aggregation process was clearly observed by 2D IR to be reversible at the early stage aggregation.