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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Cleavage of focal adhesion kinase is an early marker and modulator of oxidative stress-induced apoptosis

Cited 20 times inthomson ciCited 21 times inthomson ci
Title
Cleavage of focal adhesion kinase is an early marker and modulator of oxidative stress-induced apoptosis
Author
Mian, Md. FirozKang, ChangkeunLee, SeunghwanChoi, Jang HyunBae, Sun SikKim, Sun-HeeKim, Yun-HeeRyu, Sung HoSuh, Pann-GhillKim, Jong-ShuKim, Euikyung
Issue Date
2008-01
Publisher
ELSEVIER IRELAND LTD
Citation
CHEMICO-BIOLOGICAL INTERACTIONS, v.171, no.1, pp.57 - 66
Abstract
Focal adhesion kinase (FAK) is a signaling molecule associated with cell survival. Previously, we showed that thimerosal, a reactive oxygen species (ROS) generator, can acutely induce FAK tyrosine phosphorylation (within minutes) and chronically induce apoptosis (within days) by redox modulation in HeLa S cells. In the present study, we report that a prolonged oxidative stress by thimerosal induces a remarkable cleavage of FAK, which is accompanied with apoptosis. In fact, the kinetics of FAK cleavage has a good correlation with and actually preceding the apoptosis that was independent of anoikis. The effects were almost completely blocked by the pretreatment with either N-acetyl-L-Cysteine (ROS scavenger) or Z-VAD-FMK (pan-caspase inhibitor), suggesting ROS-induced caspase activation as a key mechanism. They could be also reproduced by hydrogen peroxide alone, which appeared to be responsible for thimerosal-mediated oxidative stress-induced apoptosis. Additionally, the down regulation of FAK with antisense oligonucleotide dramatically augmented thimerosal-induced apoptosis. We could observe similar results using human corneal epithelia] cells. Taken together, our results show that FAK is a critical cellular target of caspases during oxidative stress (particularly by hydrogen peroxide), resulting in the acceleration of subsequent apoptosis regardless of the anchorage status of cells. From the present results, it is more likely that not cell detachment but the proteolytic cleavage (or inhibition) of FAK is a key modulator as well as a promising indicator of apoptosis in epithelial cells under oxidative stress.
URI
https://scholarworks.unist.ac.kr/handle/201301/7241
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=36549018005
DOI
10.1016/j.cbi.2007.08.009
ISSN
0009-2797
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BIO_Journal Papers
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