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Park, Chan Young
Calcium Dynamics Lab
Research Interests
  • Calcium Signaling, Calcium Channels, Immune, neuroscience, Drug Development, Virus and host cell interaction.

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Direct interaction of a divergent CaM isoform and the transcription factor, MYB2, enhances salt tolerance in Arabidopsis

Cited 76 times inthomson ciCited 79 times inthomson ci
Title
Direct interaction of a divergent CaM isoform and the transcription factor, MYB2, enhances salt tolerance in Arabidopsis
Author
Yoo, JHPark, Chan YoungKim, JCHeo, WDCheong, MSPark, HCKim, MCMoon, BCChoi, MSKang, YHLee, JHKim, HSLee, SMYoon, HWLim, COYun, DJLee, SYChung, WSCho, Moo Je
Issue Date
2005-02
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.280, no.5, pp.3697 - 3706
Abstract
Calmodulin (CaM), a ubiquitous calcium-binding protein, regulates diverse cellular functions by modulating the activity of a variety of enzymes and proteins. Plants express numerous CaM isoforms that exhibit differential activation and/or inhibition of CaM-dependent enzymes in vitro. However, the specific biological functions of plant CaM are not well known. In this study, we isolated a cDNA encoding a CaM binding transcription factor, MYB2, that regulates the expression of salt- and dehydration-responsive genes in Arabidopsis. This was achieved using a salt-inducible CaM isoform (GmCaM4) as a probe from a salt-treated Arabidopsis expression library. Using domain mapping, we identified a Ca(2+)-dependent CaM binding domain in MYB2. The specific binding of CaM to CaM binding domain was confirmed by site-directed mutagenesis, a gel mobility shift assay, split ubiquitin assay, and a competition assay using a Ca(2+)/CaM-dependent enzyme. Interestingly, the specific CaM isoform GmCaM4 enhances the DNA binding activity of AtMYB2, whereas this was inhibited by a closely related CaM isoform (GmCaM1). Overexpression of GmCaM4 in Arabidopsis up-regulates the transcription rate of AtMYB2-regulated genes, including the proline-synthesizing enzyme P5CS1 (triangle(1)-pyrroline-5-carboxylate synthetase-1), which confers salt tolerance by facilitating proline accumulation. Therefore, we suggest that a specific CaM isoform mediates salt-induced Ca(2+) signaling through the activation of an MYB transcriptional activator, thereby resulting in salt tolerance in plants.
URI
https://scholarworks.unist.ac.kr/handle/201301/7191
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=13544256637
DOI
10.1074/jbc.M408237200
ISSN
0021-9258
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BIO_Journal Papers
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