BROWSE

Related Researcher

Author's Photo

Kang, Byoung Heon
Cancer Biology Lab
Research Interests
  • Mitochondrial chaperones, cancer biology, cell death, metabolism, cancer stem cells, cancer therapeutics development

ITEM VIEW & DOWNLOAD

The structure of procaspase 6 is similar to that of active mature caspase 6

Cited 16 times inthomson ciCited 15 times inthomson ci
Title
The structure of procaspase 6 is similar to that of active mature caspase 6
Author
Kang, Byoung HeonKo, EKwon, OKChoi, KY
Issue Date
2002-06
Publisher
PORTLAND PRESS LTD
Citation
BIOCHEMICAL JOURNAL, v.364, no.3, pp.629 - 634
Abstract
To investigate the structural characteristics and activation mechanism of the precursor caspase, genes encoding the inactive proform and the active mature form of caspase 6 were expressed in Escherichia coli and the proteins of both forms were purified to homogeneity. The structure of each protein was characterized by chemical cross-linking, size-exclusion chromatography, CD and fluorescence spectroscopies. The pro-form caspase 6 exhibits a dimeric structure and its overall secondary structure was found to be similar to that of the mature caspase 6. Upon the maturation of procaspase 6, the maximum fluorescence wavelength λmax was red-shifted from 330 to 337 nm and the fluorescence intensity of λmax was increased. This fluorescence spectral change indicates that the environment of a tryptophan residue in the substrate-binding site can be changed to a more polar one when the procaspase 6 is processed. Taken together, our results strongly demonstrate that precursor caspase 6 exists as a dimer and its overall structure is similar to that of the active caspase 6. Our results also suggest that the local conformational change at the substrate-binding site, with no drastic change in the overall structure, seems to enable precursor caspase 6 to become the active mature enzyme.
URI
https://scholarworks.unist.ac.kr/handle/201301/7126
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0037097012
DOI
10.1042/BJ20011787
ISSN
0264-6021
Appears in Collections:
BIO_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qrcode

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU