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Kim, Jeong Beom
Molecular Biomedicine Lab
Research Interests
  • Patient-specific stem cells, induced pluripotent stem (iPS) cells, direct conversion, 3D bio-printing, Regenerative medicine, Patient-specific drug screening

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Induction of Pluripotency in Adult Unipotent Germline Stem Cells

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Title
Induction of Pluripotency in Adult Unipotent Germline Stem Cells
Author
Ko, KinarmTapia, NataliaWu, GuangmingKim, Jeong BeomArauzo-Bravo, Marcos J.Sasse, PhilippGlaser, TamaraRuau, DavidHan, Dong WookGreber, BorisHausdoerfer, KirstenSebastiano, VittorioStehling, MartinFleischmann, Bernd K.Bruestle, OliverZenke, MartinSchoeler, Hans R.
Keywords
STEMCELL
Issue Date
2009-07
Publisher
CELL PRESS
Citation
CELL STEM CELL, v.5, no.1, pp.87 - 96
Abstract
Mouse and human stem cells with features similar to those of embryonic stem cells have been derived from testicular cells. Although pluripotent stem cells have been obtained from defined germline stem cells (GSCs) of mouse neonatal testis, only multipotent stem cells have been obtained so far from defined cells of mouse adult testis. In this study we describe a robust and reproducible protocol for obtaining germline-derived pluripotent stem (gPS) cells from adult unipotent GSCs. Pluripotency of gPS cells was confirmed by in vitro and in vivo differentiation, including germ cell contribution and transmission. As determined by clonal analyses, gPS cells indeed originate from unipotent GSCs. We propose that the conversion process requires a GSC culture microenvironment that depends on the initial number of plated GSCs and the length of culture time.
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DOI
10.1016/j.stem.2009.05.025
ISSN
1934-5909
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BME_Journal Papers
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