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Bae, Sung Chul
Multiscale Optical Imaging Lab
Research Interests
  • Multiscale optical imaging, laser spectroscopy, laser microscopy, laser sensing, soft matter physics, surface and interfacial science, laser Matter Interaction

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Vesicle Budding Induced by a Pore-Forming Peptide

Cited 28 times inthomson ciCited 29 times inthomson ci
Title
Vesicle Budding Induced by a Pore-Forming Peptide
Author
Yu, YanVroman, Julie A.Bae, Sung ChulGranick, Steve
Issue Date
2010-01
Publisher
AMER CHEMICAL SOC
Citation
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.132, no.1, pp.195 - 201
Abstract
We describe, in a system whose uniqueness is that the presence of pores allows the volume to vary as budding proceeds, how phase separation on the surface of spheres extrudes material in the process called "budding". The system is giant phospholipid vesicles (GUVs) containing phase-separated regions of DOPC (soft, liquid) and DPPC (stiff, gel), with cholesterol and without it. Budding is triggered by adding the cationic pore-forming peptide, melittin. Without cholesterol, fluorescence experiments show that melittin selectively binds to the liquid domains, inducing them to form mainly exocytotic monodisperse smaller vesicle buds of this same material, causing the parent GUV to shrink. The effect of cholesterol is to produce just a few large buds following domain coalescence, rather than numerous smaller monodisperse ones. Line tension is experimentally shown to be essential for budding in this multicomponent membrane.
URI
https://scholarworks.unist.ac.kr/handle/201301/7035
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=74849097759
DOI
10.1021/ja9059014
ISSN
0002-7863
Appears in Collections:
CHM_Journal Papers
BME_Journal Papers
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