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Lee, Sung Kuk
Synthetic Biology & Metabolic Engineering Laboratory
Research Interests
  • Biofuels production
  • Gene discovery
  • Protein engineering
  • Development of gene expression systems
  • Metabolic pathway regulation
  • Metabolic pathway optimization using functional genomics
  • Synthetic biology
  • Development of biosensors

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Directed evolution of AraC for improved compatibility of arabinose- and lactose-inducible promoters

Cited 40 times inthomson ciCited 37 times inthomson ci
Title
Directed evolution of AraC for improved compatibility of arabinose- and lactose-inducible promoters
Author
Lee, Sung KukChou, Howard H.Pfleger, Brian F.Newman, Jack D.Yoshikuni, YasuoKeasling, Jay D.
Keywords
Arabinose;  Dimerization domains;  Lactose
Issue Date
2007-09
Publisher
AMER SOC MICROBIOLOGY
Citation
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.73, no.18, pp.5711 - 5715
Abstract
Synthetic biological systems often require multiple, independently inducible promoters in order to control the expression levels of several genes; however, cross talk between the promoters limits this ability. Here, we demonstrate the directed evolution of AraC to construct an arabinose-inducible (P-BAD) system that is more compatible with IPTG (isopropyl-beta-D-1-thiogalactopyranoside) induction of a lactose-inducible (P-lac) system. The constructed system is 10 times more sensitive to arabinose and tolerates IPTG significantly better than the wild type. Detailed studies indicate that the AraC dimerization domain and C terminus are important for the increased sensitivity of AraC to arabinose.
URI
https://scholarworks.unist.ac.kr/handle/201301/6743
DOI
10.1128/AEM.00791-07
ISSN
0099-2240
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