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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 5715 | - |
dc.citation.number | 18 | - |
dc.citation.startPage | 5711 | - |
dc.citation.title | APPLIED AND ENVIRONMENTAL MICROBIOLOGY | - |
dc.citation.volume | 73 | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.contributor.author | Chou, Howard H. | - |
dc.contributor.author | Pfleger, Brian F. | - |
dc.contributor.author | Newman, Jack D. | - |
dc.contributor.author | Yoshikuni, Yasuo | - |
dc.contributor.author | Keasling, Jay D. | - |
dc.date.accessioned | 2023-12-22T09:10:50Z | - |
dc.date.available | 2023-12-22T09:10:50Z | - |
dc.date.created | 2014-09-29 | - |
dc.date.issued | 2007-09 | - |
dc.description.abstract | Synthetic biological systems often require multiple, independently inducible promoters in order to control the expression levels of several genes; however, cross talk between the promoters limits this ability. Here, we demonstrate the directed evolution of AraC to construct an arabinose-inducible (P-BAD) system that is more compatible with IPTG (isopropyl-beta-D-1-thiogalactopyranoside) induction of a lactose-inducible (P-lac) system. The constructed system is 10 times more sensitive to arabinose and tolerates IPTG significantly better than the wild type. Detailed studies indicate that the AraC dimerization domain and C terminus are important for the increased sensitivity of AraC to arabinose. | - |
dc.identifier.bibliographicCitation | APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.73, no.18, pp.5711 - 5715 | - |
dc.identifier.doi | 10.1128/AEM.00791-07 | - |
dc.identifier.issn | 0099-2240 | - |
dc.identifier.scopusid | 2-s2.0-34648828365 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/6743 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34648828365 | - |
dc.identifier.wosid | 000249550400002 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | Directed evolution of AraC for improved compatibility of arabinose- and lactose-inducible promoters | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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