A surface forces study of DNA hybridization
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- A surface forces study of DNA hybridization
- Cho, Yoon-Kyoung; Kim, S; Lim, G; Granick, Steve
- Issue Date
- AMER CHEMICAL SOC
- LANGMUIR, v.17, no.25, pp.7732 - 7734
- We report the first use of a surface forces apparatus to study the hybridization of DNA. We study the regime of very high ionic strength (≈1 M) at which commercial DNA chip operations are performed. Using a thiol end-attached single-stranded oligonucleotide, we find that exposure to the complementary strand resulted in larger thickness. In addition, the resistance to small-amplitude shear deformations when opposed to a nonadsorbing surface (mica) increased significantly after hybridization. The thickness at onset of significant shear resistance was, for single-stranded DNA, considerably less than that of hybridized DNA. This could provide a new method to detect DNA hybridization efficiency.
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