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Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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A surface forces study of DNA hybridization

Cited 24 times inthomson ciCited 24 times inthomson ci
Title
A surface forces study of DNA hybridization
Author
Cho, Yoon-KyoungKim, SLim, GGranick, Steve
Issue Date
2001-12
Publisher
AMER CHEMICAL SOC
Citation
LANGMUIR, v.17, no.25, pp.7732 - 7734
Abstract
We report the first use of a surface forces apparatus to study the hybridization of DNA. We study the regime of very high ionic strength (≈1 M) at which commercial DNA chip operations are performed. Using a thiol end-attached single-stranded oligonucleotide, we find that exposure to the complementary strand resulted in larger thickness. In addition, the resistance to small-amplitude shear deformations when opposed to a nonadsorbing surface (mica) increased significantly after hybridization. The thickness at onset of significant shear resistance was, for single-stranded DNA, considerably less than that of hybridized DNA. This could provide a new method to detect DNA hybridization efficiency.
URI
https://scholarworks.unist.ac.kr/handle/201301/5914
URL
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0035846799
DOI
10.1021/la0113769
ISSN
0743-7463
Appears in Collections:
CHM_Journal Papers
BME_Journal Papers
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