File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

조윤경

Cho, Yoon-Kyoung
FRUITS Lab.
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

A surface forces study of DNA hybridization

Author(s)
Cho, Yoon-KyoungKim, SLim, GGranick, Steve
Issued Date
2001-12
DOI
10.1021/la0113769
URI
https://scholarworks.unist.ac.kr/handle/201301/5914
Fulltext
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0035846799
Citation
LANGMUIR, v.17, no.25, pp.7732 - 7734
Abstract
We report the first use of a surface forces apparatus to study the hybridization of DNA. We study the regime of very high ionic strength (≈1 M) at which commercial DNA chip operations are performed. Using a thiol end-attached single-stranded oligonucleotide, we find that exposure to the complementary strand resulted in larger thickness. In addition, the resistance to small-amplitude shear deformations when opposed to a nonadsorbing surface (mica) increased significantly after hybridization. The thickness at onset of significant shear resistance was, for single-stranded DNA, considerably less than that of hybridized DNA. This could provide a new method to detect DNA hybridization efficiency.
Publisher
AMER CHEMICAL SOC
ISSN
0743-7463

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.