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Park, Cheol-Min
Synthetic & Medicinal Chemistry Lab
Research Interests
  • Organic synthesis, medicinal chemistry, chemical biology

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A chemical tool for blue light-inducible proximity photo-crosslinking in live cells

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Title
A chemical tool for blue light-inducible proximity photo-crosslinking in live cells
Author
Mishra, Pratyush KumarKang, Myeong-GyunLee, HakbongKim, SeungjoonChoi, SubinSharma, NirmaliPark, Cheol-MinKo, JaewonLee, ChangwookSeo, Jeong KonRhee, Hyun-Woo
Issue Date
2021-12
Publisher
Royal Society of Chemistry
Citation
CHEMICAL SCIENCE
Abstract
We developed a proximity photo-crosslinking method (Spotlight) with a 4-azido-N-ethyl-1,8-naphthalimide (AzNP) moiety that can be converted to reactive aryl nitrene species using ambient blue light-emitting diode light. Using an AzNP-conjugated HaloTag ligand (VL1), blue light-induced photo-crosslinked products of various HaloTag-conjugated proteins of interest were detected in subcellular spaces in live cells. Chemical or heat stress-induced dynamic changes in the proteome were also detected, and photo-crosslinking in the mouse brain tissue was enabled. Using Spotlight, we further identified the host interactome of SARS-CoV-2 nucleocapsid (N) protein, which is essential for viral genome assembly. Mass analysis of the VL1-crosslinked product of N-HaloTag in HEK293T cells showed that RNA-binding proteins in stress granules were exclusively enriched in the cross-linked samples. These results tell that our method can reveal the interactome of protein of interest within a short distance in live cells.
URI
https://scholarworks.unist.ac.kr/handle/201301/55891
URL
https://pubs.rsc.org/en/content/articlelanding/2022/SC/D1SC04871F
DOI
10.1039/d1sc04871f
ISSN
2041-6520
Appears in Collections:
CHM_Journal Papers
BIO_Journal Papers
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