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Park, Chan Young
Calcium Dynamics Lab.
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Simultaneous Real-Time Three-Dimensional Localization and FRET Measurement of Two Distinct Particles

Author(s)
Chen, XingxiangLiu, TengQin, XiananQuang Quan NguyenLee, Sang KwonLee, ChanwooRen, YaguangChu, JunZhu, GuangYoon, Tae-YoungPark, Chan YoungPark, Hyokeun
Issued Date
2021-09
DOI
10.1021/acs.nanolett.1c01328
URI
https://scholarworks.unist.ac.kr/handle/201301/54630
Fulltext
https://pubs.acs.org/doi/10.1021/acs.nanolett.1c01328
Citation
NANO LETTERS, v.21, no.18, pp.7479 - 7485
Abstract
Many biological processes employ mechanisms involving the locations and interactions of multiple components. Given that most biological processes occur in three dimensions, the simultaneous measurement of three-dimensional locations and interactions is necessary. However, the simultaneous three-dimensional precise localization and measurement of interactions in real time remains challenging. Here, we report a new microscopy technique to localize two spectrally distinct particles in three dimensions with an accuracy (2.35 sigma) of tens of nanometers with an exposure time of 100 ms and to measure their real-time interactions using fluorescence resonance energy transfer (FRET) simultaneously. Using this microscope, we tracked two distinct vesicles containing t-SNAREs or v-SNARE in three dimensions and observed FRET simultaneously during single-vesicle fusion in real time, revealing the nanoscale motion and interactions of single vesicles in vesicle fusion. Thus, this study demonstrates that our microscope can provide detailed information about real-time three-dimensional nanoscale locations, motion, and interactions in biological processes.
Publisher
AMER CHEMICAL SOC
ISSN
1530-6984
Keyword (Author)
single-particle trackingFRETnanometric precisionthree-dimensional localizationvesicle fusion
Keyword
SINGLE-MOLECULE FLUORESCENCERESONANCE ENERGY-TRANSFERIN-VITROTRACKINGMICROSCOPY

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