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Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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New Multiscale Characterization Methodology for Effective Determination of Isolation-Structure-Function Relationship of Extracellular Vesicles

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Title
New Multiscale Characterization Methodology for Effective Determination of Isolation-Structure-Function Relationship of Extracellular Vesicles
Author
Phan, Thanh HuyenDivakarla, Shiva KaminiYeo, Jia HaoLei, QingyuTharkar, PriyankaPansani, Taisa NogueiraLeslie, Kathryn G.Tong, MaggieColeman, Victoria A.Jamting, AsaDu Plessis, Mar-DeanNew, Elizabeth J.Kalionis, BillDemokritou, PhilipWoo, Hyun-KyungCho, Yoon-KyoungChrzanowski, Wojciech
Issue Date
2021-06
Publisher
FRONTIERS MEDIA SA
Citation
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v.9, pp.669537
Abstract
Extracellular vesicles (EVs) have been lauded as next-generation medicines, but very few EV-based therapeutics have progressed to clinical use. Limited clinical translation is largely due to technical barriers that hamper our ability to mass produce EVs, i.e., to isolate, purify, and characterize them effectively. Technical limitations in comprehensive characterization of EVs lead to unpredicted biological effects of EVs. Here, using a range of optical and non-optical techniques, we showed that the differences in molecular composition of EVs isolated using two isolation methods correlated with the differences in their biological function. Our results demonstrated that the isolation method determines the composition of isolated EVs at single and sub-population levels. Besides the composition, we measured for the first time the dry mass and predicted sedimentation of EVs. These parameters were likely to contribute to the biological and functional effects of EVs on single cell and cell cultures. We anticipate that our new multiscale characterization approach, which goes beyond traditional experimental methodology, will support fundamental understanding of EVs as well as elucidate the functional effects of EVs in in vitro and in vivo studies. Our findings and methodology will be pivotal for developing optimal isolation methods and establishing EVs as mainstream therapeutics and diagnostics. This innovative approach is applicable to a wide range of sectors including biopharma and biotechnology as well as to regulatory agencies.
URI
https://scholarworks.unist.ac.kr/handle/201301/53318
URL
https://www.frontiersin.org/articles/10.3389/fbioe.2021.669537/full
DOI
10.3389/fbioe.2021.669537
ISSN
2296-4185
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BME_Journal Papers
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