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Cho, Yoon-Kyoung
FRUITS Lab.
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dc.citation.startPage 669537 -
dc.citation.title FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY -
dc.citation.volume 9 -
dc.contributor.author Phan, Thanh Huyen -
dc.contributor.author Divakarla, Shiva Kamini -
dc.contributor.author Yeo, Jia Hao -
dc.contributor.author Lei, Qingyu -
dc.contributor.author Tharkar, Priyanka -
dc.contributor.author Pansani, Taisa Nogueira -
dc.contributor.author Leslie, Kathryn G. -
dc.contributor.author Tong, Maggie -
dc.contributor.author Coleman, Victoria A. -
dc.contributor.author Jamting, Asa -
dc.contributor.author Du Plessis, Mar-Dean -
dc.contributor.author New, Elizabeth J. -
dc.contributor.author Kalionis, Bill -
dc.contributor.author Demokritou, Philip -
dc.contributor.author Woo, Hyun-Kyung -
dc.contributor.author Cho, Yoon-Kyoung -
dc.contributor.author Chrzanowski, Wojciech -
dc.date.accessioned 2023-12-21T15:42:38Z -
dc.date.available 2023-12-21T15:42:38Z -
dc.date.created 2021-07-13 -
dc.date.issued 2021-06 -
dc.description.abstract Extracellular vesicles (EVs) have been lauded as next-generation medicines, but very few EV-based therapeutics have progressed to clinical use. Limited clinical translation is largely due to technical barriers that hamper our ability to mass produce EVs, i.e., to isolate, purify, and characterize them effectively. Technical limitations in comprehensive characterization of EVs lead to unpredicted biological effects of EVs. Here, using a range of optical and non-optical techniques, we showed that the differences in molecular composition of EVs isolated using two isolation methods correlated with the differences in their biological function. Our results demonstrated that the isolation method determines the composition of isolated EVs at single and sub-population levels. Besides the composition, we measured for the first time the dry mass and predicted sedimentation of EVs. These parameters were likely to contribute to the biological and functional effects of EVs on single cell and cell cultures. We anticipate that our new multiscale characterization approach, which goes beyond traditional experimental methodology, will support fundamental understanding of EVs as well as elucidate the functional effects of EVs in in vitro and in vivo studies. Our findings and methodology will be pivotal for developing optimal isolation methods and establishing EVs as mainstream therapeutics and diagnostics. This innovative approach is applicable to a wide range of sectors including biopharma and biotechnology as well as to regulatory agencies. -
dc.identifier.bibliographicCitation FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v.9, pp.669537 -
dc.identifier.doi 10.3389/fbioe.2021.669537 -
dc.identifier.issn 2296-4185 -
dc.identifier.scopusid 2-s2.0-85108378696 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/53318 -
dc.identifier.url https://www.frontiersin.org/articles/10.3389/fbioe.2021.669537/full -
dc.identifier.wosid 000663751800001 -
dc.language 영어 -
dc.publisher FRONTIERS MEDIA SA -
dc.title New Multiscale Characterization Methodology for Effective Determination of Isolation-Structure-Function Relationship of Extracellular Vesicles -
dc.type Article -
dc.description.isOpenAccess TRUE -
dc.relation.journalWebOfScienceCategory Biotechnology & Applied Microbiology; Multidisciplinary Sciences -
dc.relation.journalResearchArea Biotechnology & Applied Microbiology; Science & Technology - Other Topics -
dc.type.docType Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor extracellular vesicles -
dc.subject.keywordAuthor mesenchymal stromal -
dc.subject.keywordAuthor stem cell -
dc.subject.keywordAuthor isolation methods -
dc.subject.keywordAuthor ultracentrifugation -
dc.subject.keywordAuthor tangential flow filtration -
dc.subject.keywordAuthor nanodosimetry -
dc.subject.keywordAuthor single vesicle analysis -
dc.subject.keywordAuthor exosomes -
dc.subject.keywordPlus NITRIC-OXIDE -
dc.subject.keywordPlus QUANTIFICATION -
dc.subject.keywordPlus MICRORNA -
dc.subject.keywordPlus EXOSOMES -

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