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Kim, Donghyuk
Systems Biology Lab
Research Interests
  • Systems Biology, Computational Biology, Bacterial Pathogens, Host-Pathogen Interaction, Anti-Microbial Resistance

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Enhanced production of nonanedioic acid from nonanoic acid by engineered Escherichia coli

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Title
Enhanced production of nonanedioic acid from nonanoic acid by engineered Escherichia coli
Author
Lee, YongjooSathesh-Prabu, ChandranKwak, Geun HwaBang, InaJung, Hyun WookKim, DonghyukLee, Sung Kuk
Issue Date
2021-05
Publisher
WILEY-V C H VERLAG GMBH
Citation
BIOTECHNOLOGY JOURNAL, pp.e2000416
Abstract
In this study, whole-cell biotransformation was conducted to produce nonanedioic acid from nonanoic acid by expressing the alkane hydroxylating system (AlkBGT) from Pseudomonas putida GPo1 in Escherichia coli. Following adaptive laboratory evolution, an efficient E. coli mutant strain, designated as MRE, was successfully obtained, demonstrating the fastest growth (27-fold higher) on nonanoic acid as the sole carbon source compared to the wild-type strain. Additionally, the MRE strain was engineered to block nonanoic acid degradation by deleting fadE. The resulting strain exhibited a 12.8-fold increase in nonanedioic acid production compared to the wild-type strain. Six mutations in acrR, P-crp, dppA, P-fadD, e14, and yeaR were identified in the mutant MRE strain, which was characterized using genomic modifications and RNA-sequencing. The acquired mutations were found to be beneficial for rapid growth and nonanedioic acid production.
URI
https://scholarworks.unist.ac.kr/handle/201301/53001
URL
https://onlinelibrary.wiley.com/doi/10.1002/biot.202000416
DOI
10.1002/biot.202000416
ISSN
1860-6768
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