There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.number | 3 | - |
dc.citation.startPage | e2000416 | - |
dc.citation.title | BIOTECHNOLOGY JOURNAL | - |
dc.citation.volume | 17 | - |
dc.contributor.author | Lee, Yongjoo | - |
dc.contributor.author | Sathesh-Prabu, Chandran | - |
dc.contributor.author | Kwak, Geun Hwa | - |
dc.contributor.author | Bang, Ina | - |
dc.contributor.author | Jung, Hyun Wook | - |
dc.contributor.author | Kim, Donghyuk | - |
dc.contributor.author | Lee, Sung Kuk | - |
dc.date.accessioned | 2023-12-21T14:37:16Z | - |
dc.date.available | 2023-12-21T14:37:16Z | - |
dc.date.created | 2021-06-08 | - |
dc.date.issued | 2022-03 | - |
dc.description.abstract | In this study, whole-cell biotransformation was conducted to produce nonanedioic acid from nonanoic acid by expressing the alkane hydroxylating system (AlkBGT) from Pseudomonas putida GPo1 in Escherichia coli. Following adaptive laboratory evolution, an efficient E. coli mutant strain, designated as MRE, was successfully obtained, demonstrating the fastest growth (27-fold higher) on nonanoic acid as the sole carbon source compared to the wild-type strain. Additionally, the MRE strain was engineered to block nonanoic acid degradation by deleting fadE. The resulting strain exhibited a 12.8-fold increase in nonanedioic acid production compared to the wild-type strain. Six mutations in acrR, P-crp, dppA, P-fadD, e14, and yeaR were identified in the mutant MRE strain, which was characterized using genomic modifications and RNA-sequencing. The acquired mutations were found to be beneficial for rapid growth and nonanedioic acid production. | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY JOURNAL, v.17, no.3, pp.e2000416 | - |
dc.identifier.doi | 10.1002/biot.202000416 | - |
dc.identifier.issn | 1860-6768 | - |
dc.identifier.scopusid | 2-s2.0-85106689730 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/53001 | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/biot.202000416 | - |
dc.identifier.wosid | 000655515400001 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Enhanced production of nonanedioic acid from nonanoic acid by engineered Escherichia coli | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods; Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | adaptive laboratory evolution | - |
dc.subject.keywordAuthor | biotransformation | - |
dc.subject.keywordAuthor | medium chain fatty acids | - |
dc.subject.keywordAuthor | nonanedioic acid | - |
dc.subject.keywordAuthor | nonanoic acid | - |
dc.subject.keywordPlus | CHAIN FATTY-ACIDS | - |
dc.subject.keywordPlus | PSEUDOMONAS-PUTIDA GPO1 | - |
dc.subject.keywordPlus | DIPEPTIDE PERMEASE | - |
dc.subject.keywordPlus | LONG | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | OXYFUNCTIONALIZATION | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | EFFLUX | - |
dc.subject.keywordPlus | GENES | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.