File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

조형준

Cho, Hyungjoon
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Target-switchable Gd(III)-DOTA/protein cage nanoparticle conjugates with multiple targeting affibody molecules as target selective T1 contrast agents for high-field MRI

Author(s)
Kim, HansolJin, SeokhaChoi, HyukjunKang, MungsooPark SeongGukJun, HeejinCho, HyungjoonKang, Sebyung
Issued Date
2021-07
DOI
10.1016/j.jconrel.2021.05.029
URI
https://scholarworks.unist.ac.kr/handle/201301/52941
Fulltext
https://www.sciencedirect.com/science/article/pii/S0168365921002534?via%3Dihub
Citation
JOURNAL OF CONTROLLED RELEASE, v.335, pp.269 - 280
Abstract
Magnetic resonance imaging (MRI) is a non-invasive in vivo imaging tool, providing high enough spatial resolution to obtain both the anatomical and the physiological information of patients. However, MRI generally suffers from relatively low sensitivity often requiring the aid of contrast agents (CA) to enhance the contrast of vessels and/or the tissues of interest from the background. The targeted delivery of diagnostic probes to the specific lesion is a powerful approach for early diagnosis and signal enhancement leading to the effective treatment of various diseases. Here, we established targeting ligand switchable nanoplatforms using lumazine synthase protein cage nanoparticles derived from Aquifex aeolicus (AaLS) by genetically introducing the SpyTag peptide (ST) to the C-terminus of the AaLS subunits to form an ST-displaying AaLS (AaLS-ST). Conversely, multiple targeting ligands were constructed by genetically fusing SpyCatcher protein (SC) to either HER2 or EGFR targeting affibody molecules (SC-HER2Afb or SC-EGFRAfb). Gd(III)-DOTA complexes were chemically attached to the AaLS-ST and the external surface of the Gd(III)-DOTA conjugated AaLS-ST (Gd(III)-DOTA-AaLSST) were successfully decorated with either the HER2Afb or the EGFRAfb. The resulting Gd(III)-DOTA-AaLS/ HER2Afb and Gd(III)-DOTA-AaLS/EGFR2Afb exhibited high r1 relaxivity values of 57 and 25 mM-1 s-1 at 1.4 and 7 T, respectively, which were 10-fold or higher than those of the clinically used Dotarem. Their targetselective contrast enhancements were confirmed with in vitro cell-based MRI scans and the in vivo MR imaging of tumor-bearing mouse models at 7 T. A target-switchable AaLS-based nanoplatform that was developed in this study might serve as a promising T1 CA developing platform at a high magnetic field to detect various tumor sites in a target-specific manner in future clinical applications.
Publisher
ELSEVIER
ISSN
0168-3659
Keyword (Author)
Protein cage nanoparticlesMRIContrast agent7 TTarget switchable
Keyword
BLOOD-BRAIN-BARRIERP22 VIRAL CAPSIDSMAGNETIC-FIELDIRON-OXIDEGADOLINIUMRELAXIVITYPROTEINAPOFERRITINDISRUPTIONEXPRESSION

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.