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Auxotrophic Selection Strategy for Improved Production of Coenzyme B-12 in Escherichia coli

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Title
Auxotrophic Selection Strategy for Improved Production of Coenzyme B-12 in Escherichia coli
Author
Noh, Myung HyunLim, Hyun GyuMoon, DaeyeongPark, SunghoonJung, Gyoo Yeol
Issue Date
2020-03
Publisher
CELL PRESS
Citation
ISCIENCE, v.23, no.3, pp.UNSP 10089
Abstract
The production of coenzyme B-12 using well-characterized microorganisms, such as Escherichia coli, has recently attracted considerable attention to meet growing demands of coenzyme B-12 in various applications. In the present study, we designed an auxotrophic selection strategy and demonstrated the enhanced production of coenzyme B-12 using a previously engineered coenzyme B-12-producing E. coli strain. To select a high producer, the coenzyme B-12-independent methionine synthase (metE) gene was deleted in E. coli, thus limiting its methionine synthesis to only that via coenzyme B-12-dependent synthase (encoded by metH). Following the deletion of metE, significantly enhanced production of the specific coenzyme B-12 validated the coenzyme B-12-dependent auxotrophic growth. Further precise tuning of the auxotrophic system by varying the expression of metH substantially increased the cell biomass and coenzyme B-12 production, suggesting that our strategy could be effectively applied to E. coli and other coenzyme B-12-producing strains.
URI
https://scholarworks.unist.ac.kr/handle/201301/49311
URL
https://www.sciencedirect.com/science/article/pii/S2589004220300742
DOI
10.1016/j.isci.2020.100890
ISSN
2589-0042
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ECHE_Journal Papers
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