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Park, Sunghoon
Biochemical Engineering Lab.
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dc.citation.number 3 -
dc.citation.startPage UNSP 10089 -
dc.citation.title ISCIENCE -
dc.citation.volume 23 - Noh, Myung Hyun - Lim, Hyun Gyu - Moon, Daeyeong - Park, Sunghoon - Jung, Gyoo Yeol - 2023-12-21T17:46:38Z - 2023-12-21T17:46:38Z - 2020-06-29 - 2020-03 -
dc.description.abstract The production of coenzyme B-12 using well-characterized microorganisms, such as Escherichia coli, has recently attracted considerable attention to meet growing demands of coenzyme B-12 in various applications. In the present study, we designed an auxotrophic selection strategy and demonstrated the enhanced production of coenzyme B-12 using a previously engineered coenzyme B-12-producing E. coli strain. To select a high producer, the coenzyme B-12-independent methionine synthase (metE) gene was deleted in E. coli, thus limiting its methionine synthesis to only that via coenzyme B-12-dependent synthase (encoded by metH). Following the deletion of metE, significantly enhanced production of the specific coenzyme B-12 validated the coenzyme B-12-dependent auxotrophic growth. Further precise tuning of the auxotrophic system by varying the expression of metH substantially increased the cell biomass and coenzyme B-12 production, suggesting that our strategy could be effectively applied to E. coli and other coenzyme B-12-producing strains. -
dc.identifier.bibliographicCitation ISCIENCE, v.23, no.3, pp.UNSP 10089 -
dc.identifier.doi 10.1016/j.isci.2020.100890 -
dc.identifier.issn 2589-0042 -
dc.identifier.scopusid 2-s2.0-85079669915 -
dc.identifier.uri -
dc.identifier.url -
dc.identifier.wosid 000528358900013 -
dc.language 영어 -
dc.publisher CELL PRESS -
dc.title Auxotrophic Selection Strategy for Improved Production of Coenzyme B-12 in Escherichia coli -
dc.type Article -
dc.description.isOpenAccess TRUE -
dc.relation.journalWebOfScienceCategory Multidisciplinary Sciences -
dc.relation.journalResearchArea Science & Technology - Other Topics -
dc.type.docType Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordPlus REAL-TIME PCR -
dc.subject.keywordPlus COMBINATORIAL DESIGN -
dc.subject.keywordPlus MICROBIAL-PRODUCTION -
dc.subject.keywordPlus VITAMIN-B-12 -
dc.subject.keywordPlus METHIONINE -
dc.subject.keywordPlus PROTEIN -
dc.subject.keywordPlus OPTIMIZATION -
dc.subject.keywordPlus EVOLUTION -
dc.subject.keywordPlus ACETATE -
dc.subject.keywordPlus GENES -


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