Full metadata record
DC Field | Value | Language |
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dc.citation.number | 3 | - |
dc.citation.startPage | UNSP 10089 | - |
dc.citation.title | ISCIENCE | - |
dc.citation.volume | 23 | - |
dc.contributor.author | Noh, Myung Hyun | - |
dc.contributor.author | Lim, Hyun Gyu | - |
dc.contributor.author | Moon, Daeyeong | - |
dc.contributor.author | Park, Sunghoon | - |
dc.contributor.author | Jung, Gyoo Yeol | - |
dc.date.accessioned | 2023-12-21T17:46:38Z | - |
dc.date.available | 2023-12-21T17:46:38Z | - |
dc.date.created | 2020-06-29 | - |
dc.date.issued | 2020-03 | - |
dc.description.abstract | The production of coenzyme B-12 using well-characterized microorganisms, such as Escherichia coli, has recently attracted considerable attention to meet growing demands of coenzyme B-12 in various applications. In the present study, we designed an auxotrophic selection strategy and demonstrated the enhanced production of coenzyme B-12 using a previously engineered coenzyme B-12-producing E. coli strain. To select a high producer, the coenzyme B-12-independent methionine synthase (metE) gene was deleted in E. coli, thus limiting its methionine synthesis to only that via coenzyme B-12-dependent synthase (encoded by metH). Following the deletion of metE, significantly enhanced production of the specific coenzyme B-12 validated the coenzyme B-12-dependent auxotrophic growth. Further precise tuning of the auxotrophic system by varying the expression of metH substantially increased the cell biomass and coenzyme B-12 production, suggesting that our strategy could be effectively applied to E. coli and other coenzyme B-12-producing strains. | - |
dc.identifier.bibliographicCitation | ISCIENCE, v.23, no.3, pp.UNSP 10089 | - |
dc.identifier.doi | 10.1016/j.isci.2020.100890 | - |
dc.identifier.issn | 2589-0042 | - |
dc.identifier.scopusid | 2-s2.0-85079669915 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/49311 | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S2589004220300742 | - |
dc.identifier.wosid | 000528358900013 | - |
dc.language | 영어 | - |
dc.publisher | CELL PRESS | - |
dc.title | Auxotrophic Selection Strategy for Improved Production of Coenzyme B-12 in Escherichia coli | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | REAL-TIME PCR | - |
dc.subject.keywordPlus | COMBINATORIAL DESIGN | - |
dc.subject.keywordPlus | MICROBIAL-PRODUCTION | - |
dc.subject.keywordPlus | VITAMIN-B-12 | - |
dc.subject.keywordPlus | METHIONINE | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | OPTIMIZATION | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | ACETATE | - |
dc.subject.keywordPlus | GENES | - |
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