Silencing of TonEBP/NFAT5 transcriptional activator by RNA interference
Cited 40 times inCited 41 times in
- Silencing of TonEBP/NFAT5 transcriptional activator by RNA interference
- Na, KY; Woo, SK; Lee, SD; Kwon, H. Moo
- ENHANCER-BINDING PROTEIN; GENE-TRANSCRIPTION; TONICITY; NFAT
- Issue Date
- AMER SOC NEPHROLOGY
- JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, v.14, no.2, pp.283 - 288
- TonEBP is a transcriptional activator that is expressed throughout development in many tissues and cell types. In the kidney medulla, TonEBP appears to be an important local regulator of differentiation by virtue of stimulating several genes. To study the function of TonEBP, two small interfering RNA (siRNA) duplexes were developed that reduced TonEBP expression effectively via RNA interference. The silencing lasted only 3 d after introduction of the TonEBP-siRNA's. As expected, TonEBP-driven reporter gene expression and expression of the sodium/myo-inositol cotransproter (SMIT), aldose reductase (AR) and heat shock protein 70 (HSP70) mRNA were significantly decreased in cells where TonEBP expression was silenced. These data provide direct evidence that the SMIT, AR, and HSP70 genes are targets of TonEBP, although the potential role of other proteins, such as accessory proteins, cannot be excluded. The TonEBP-siRNA is an effective tool that should prove useful in the investigation of loss-of-function relationship in cells.
- ; Go to Link
- Appears in Collections:
- BME_Journal Papers
- Files in This Item:
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.